PR-488
UK Nursery and Landscape ProgramFaculty, Staff, and Student Cooperators |
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HorticultureFacultyRobert Anderson Technical/Professional StaffShari Dutton Farm StaffDarrell Slone StudentsSteve Berberich |
Agricultural EconomicsFacultyTim Woods Biosystems and Agricultural EngineeringFacultyRichard Gates AgronomyFacultyTim Phillips EntomologyFacultyDaniel Potter Technical StaffDavid Held StudentsJamee Hubbard |
Plant PathologyFacultyJohn Hartman Technical StaffPaul A. Bachi StudentsClaudia Cotton UK ArboretumMarcia Farris (Director) |
This is a progress report and may not reflect exactly the final outcome of ongoing projects. Therefore, please do not reproduce project reports for distribution without permission of the authors.
Mention or display of a trademark, proprietary product, or firm in text or figures does not constitute an endorsement and does not imply approval to the exclusion of other suitable products or firms.
Dewayne Ingram, Chair
The faculty, staff, and students in the UK Nursery and Landscape Program are pleased to offer this 2002 Research Report. This is one way we share information generated from a coordinated research program involving teams of faculty, staff, and students from several departments in the College of Agriculture. The report has been organized according to our primary areas of emphasis: production and economics, pest management, and plant evaluation. These areas reflect stated industry needs, expertise available at UK, and the nature of research projects around the world generating information applicable to Kentucky. If you have questions or suggestions about a particular research project, please do not hesitate to contact us.
Although the purpose of this publication is to report research results, we have also highlighted below some of our Extension programs and undergraduate and graduate degree programs that are addressing the needs of the nursery/landscape industries.
Our statewide and area educational conferences and seminars are probably the most visible activities of our Extension programs targeted to Kentucky's nursery and landscape industry. Publications, videos, slide sets, newsletters, articles in state and national industry magazines, newspaper articles, radio spots, and television programs are also important, visible elements of our Extension program. However, we are also engaged in a wide range of less visible but vital activities. More subtle activities include training for County Extension Agents so they can more effectively serve our clientele, the Plant Disease Diagnostic Clinic, soil testing and interpretative services, and diagnosis and problem-solving services. We were delighted to see that the outreach capacity of the arboretum on the UK campus increased significantly this year with the opening of the new visitors' center.
Although there are many facets to the Extension program conducted by the team of subject-matter specialists and county agents, program expansion provided through a Kentucky Horticulture Council grant from the Agriculture Development Board (tobacco settlement) funds is highlighted this year. Although most of the initial grant has been utilized to support expanded acreage of vegetables required by the three new marketing cooperatives, we were able to obtain an Extension Associate position for Nursery Crops in the western portion of the state. Amy Fulcher, who was a County Extension Agent for Horticulture in Hopkins County, has been hired in the position. Amy is a Western Kentucky University graduate and received an M.S. degree from North Carolina State University in nursery crop production. Amy is working in concert with Dr. Dunwell to provide additional support of the county agents and nursery managers and employees in the area. They have held several workshops and demonstrations and have established a demonstration pot-in-pot system at the UK Research and Education Center in Princeton. We are grateful for the additional funds to help us serve the nursery industry. The Kentucky Horticulture Council will be submitting a second proposal to the Agricultural Development Board for continuation of projects initiated this year and to enhance our ability to support the nursery and greenhouse industries.
The department offers areas of emphasis in Horticultural Enterprise Management and Horticultural Science within a Plant and Soil Science Bachelor of Science degree. Following are a few highlights of our undergraduate program in 2002:
The Plant and Soil Science degree program has over 120 students in the fall semester of 2002, of which almost one half are horticulture students and another one-third are turfgrass students. Eleven horticulture students graduated in 2002.
We believe that a significant portion of an undergraduate education in horticulture must come outside the classroom. In addition to the local activities of the Horticulture Club and field trips during course laboratories, students have excellent off-campus learning experiences. Here are the highlights of such opportunities in 2002.
The demand for graduates with M.S. or Ph.D. degrees in Horticulture, Entomology, Plant Pathology, Agricultural Economics, and Agricultural Engineering is high. Our M.S. graduates are being employed in the industry, Cooperative Extension Service, secondary and postsecondary education, and governmental agencies. Last year, there were nine graduate students in these degree programs conducting research directly related to the Kentucky nursery and landscape industry.
Graduate students are active participants in the UK Nursery and Landscape research program and contribute significantly to our ability to address problems and opportunities important to the Kentucky nursery and landscape industry. For example, graduate students presented research results at the Southern Nursery Association's Research Conference in Atlanta and the International Horticulture Congress in Toronto, and several will present their work during a session of the 2003 Kentucky Landscape Industry Conference and Trade Show.
In the early 80s, the Horticulture Department realized that many of our graduates lacked exposure to the range of horticulture practices outside Ken tucky. The faculty made a commitment to provide at least one study tour opportunity per year to our students. Fulfillment of that commitment has primarily been through Dr. McNiel, who has often covered his travel expenses personally. He has led educational tours of industries and gardens throughout Kentucky, the United States, Europe, New Zealand, and China. Dr. McNiel has been a local, regional, and national leader for the Associated Landscape Contractors of America. He has assisted in their Student Career Days and has hosted this national event several times.
The Robert E. McNiel Horticulture Enrichment Fund is being endowed to honor Dr. McNiel and to provide support for faculty and student travel on our study tours. Dr. McNiel will be retiring within the next five years, and this is our opportunity to support future students wishing to participate in educational tours and activities. These study tours allow students to compare technology development at leading horticultural sites and research centers with application to horticulture in Kentucky and to determine the applicability of this technology to the Kentucky horticultural industries.
We are taking advantage of a unique opportunity through Kentucky's Research Challenge Trust Fund (RCTF). Any gift to this fund, or pledge made for payment over a five-year period, will be matched on a dollar-for-dollar basis. However, in order to be eligible for the match, we must have a minimum of $50,000 in gifts and/or pledges. As a result of UK's hosting of the Associated Landscape Contractors of America (ALCA) Student Career Days in 1999, there is a balance of $25,000, which ALCA has endorsed using for this effort. Therefore, we must raise $25,000 to match the $25,000 we already have in order to gain the RCTF match to create a permanent endowment of $100,000. Reaching the $50,000 level is crucial, or we lose the $50,000 RCTF match. We need your help. Please consider the opportunity to provide lasting support of our students and their education. Additional information is available by contacting me in the Horticulture Department (257-1601) or by calling the College of Agriculture Development Office (859-257-7200).
Robert E. McNiel and Steve Elkins, Department of Horticulture
Aesculus parviflora (bottlebrush buckeye) has made many recommended lists during recent times. However, few plants are available on a regular basis in the nursery trade. Seed was the main method of propagation until the 1990s when Bir and Barnes (2) established a protocol for cutting propagation. Fordham (4), in his discussion of propagation of bottlebrush buckeye, devoted his explanation to seed, except for a final comment that root cuttings and root suckers can be a source. Seed availability, timing, or facilities may still limit this plant from being propagated in significant numbers by either seed or cuttings.
Layering has been recommended as a form of propagation for plants forming suckers by several authors during the 1900s (1,8). While addressing layering in one form or another, neither Mahlstede and Haber (7), Macdonald (6), Dirr and Heuser (3), nor Hartman et al. (5) define layering as a technique for bottlebrush buckeye. Bailey (1) addresses the benefits of wounding during the layering process. As a means of producing large numbers of bottlebrush buckeye with limited facilities and less dependence upon timing, we looked at mound layering. Aesculus parviflora were planted on the University of Kentucky Horticulture Research Farm during the early 1990s in north/south rows. During 1998 the plants were bush-hogged to the ground. Multi-stem regrowth occurred during 1999 and 2000. In August 2000 research was initiated in order to determine if rapid propagation could occur by mound layering Aesculus parviflora. Sawdust was row mounded 18 inches deep and 3 feet wide around 41 plants. Starting in August 2000, three stems on 10 randomly selected plants were treated on a monthly basis. Treatments included cutting into non-rooting one- or two-year old stems near the base, treating with No. 3 Hormex, and keeping the stem gapped with a section of toothpick. A drip irrigation system was installed in the plot and scheduled to run 20 minutes twice a day at 9:00 a.m. and 2:00 p.m. One-GPH emitters were spaced every 2 feet along 2-inch diameter lines. Irrigation was turned off during the dormant months.
During March 2001, plants treated each of the previous months were evaluated for rooting. Plants treated August 2000 had roots formed at the wound site on 29 of 30 stems. Plants treated September 2000 had roots formed at the wound site on eight of 30 stems. No roots were found on stems treated during October through February. During November 2001, plants were again evaluated for rooting. Rooting had occurred on all plants treated through May 2001 (Table 1). The tendency was for more stems rooting (99%) for months (Aug., Sept., April, May) when treatments were on plants that were in active growth than when treated plants (84%) were in their dormant period (Oct. through March). A plant was left untreated, and during March 2001 three plants were completely pruned back to within 3 inches of the ground for comparison to the treated plants. At the November 2001 harvest time, the unpruned plant had 14 stems that were rooted, and the three pruned plants generated a total of 68 rooted stems on current season growth. No other wounding or hormone treatment occurred on these four plants. Stems on these plants rooted with the sawdust treatment of mound layering and irrigation. The other 37 original plants were also producing new stems during 2001. Between untreated old growth stems and new growth stems, an additional 617 rooted stems were removed from these 37 plants; an average of 16.7 rooted stems per plant.
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Table 1.Stems rooted and successfully established as a liner during month-by-month treatment. |
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Month |
Rooted Stems |
Survival in the Liner Stage |
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August , 00 |
30 |
28 |
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September, 00 |
30 |
25 |
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October, 00 |
25 |
22 |
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November, 00 |
26 |
19 |
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December, 00 |
25 |
19 |
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January, 01 |
27 |
16 |
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February, 01 |
25 |
23 |
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March, 01 |
23 |
21 |
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April, 01 |
30 |
27 |
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May, 01 |
29 |
24 |
Rooted stems had either new coarse or fine roots. Coarse roots were most common, and it was suspected that stems with fine roots might not survive. This was not tracked as to root type, but survival of rooted stems as liners was recorded. Rooted stems were placed in 3-quart containers and overwintered in an unheated Quonset house. Eighty-three percent of the stems from treated plants leafed out and developed into the liner stage (Table 1). Ninety-three percent of the stems from untreated plants leafed out and developed into the liner stage.
Rapid propagation of Aesculus parviflora through mound layering is very feasible. Mound layering without wounding and hormone treatment will generate rooted shoots. Stems that do not root under normal mound layering techniques will benefit from wounding and hormone treatment.
Robert E. McNiel and Kirk Ranta, Department of Horticulture
Aesculus parviflora (bottlebrush buckeye) has been awarded elite status by being named to several outstanding-plant lists or to state plant-recognition programs. Individual plants displayed in retail settings have not always had comparable sales appeal. Instead of irregular or tall lanky growth, it was thought that lower branched and more uniform plants would be more acceptable by the buying public. Research was established to evaluate stem number, placement, and length as influenced by pruning plants during production. Seeds were collected from Aesculus parviflora and planted on the University of Kentucky Horticulture Research Farm during the fall of 2000. The resulting seedlings (6 to 30 inches tall) were harvested in November 2001 and individually placed in 3-quart containers. Plants were overwintered in a 13 x 48-foot unheated Quonset house covered with opaque poly. During February 2002, 130 plants were divided into three groups of 40+ plants. Three treatments consisted of unpruned stems, stems cut back to within 2 inches of the substrate line, and stems cut back to within 6 inches of the substrate line. Data were analyzed by analysis of variance using the General Linear Models Procedure (SAS).
During June 2002, data were collected as new stem counts originating from two positions on the remaining plant: originating above substrate line or from the base or below the substrate line.
The average number of shoots per plant was determined by averaging the count from two positions on the plant (above and below the substrate line) (Table 1). Unpruned plants showed apical dominance within the population. This resulted in the fewest shoots per plant (0.81) as many terminal buds continued to elongate without producing many additional shoots either above or below the substrate line. Pruning encouraged additional bud break, whether pruned at 2 or 6 inches. Plants pruned at 6 inches had more of the stem remaining and thus had more buds. This yielded more total shoots (1.97) than plants pruned at 2 inches (1.58) (Table 1).
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Table 1. Average total number of shoots per plant (including above and below the substrate counts) and average length of those shoots for three pruning treatments on Aesculus parviflora. |
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Pruning Treatment |
Number of Shoots y |
Average Length of Shoots z (in.) |
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Unpruned |
0.81 C |
5.17 A |
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Pruned at 2 inches |
1.58 B |
5.93 A |
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Pruned at 6 inches |
1.97 A |
6.20 A |
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y Means with the same letter for each variable are similar at p ≤ 0.01; n = 260. |
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z Means with the same letter for each variable are similar at p ≤ 0.01; n = 182. |
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Plants pruned at 2 inches produced more shoots below the substrate line (1.90) than above the substrate line (1.26) (Table 3). Plants pruned at 6 inches produced more shoots from above the substrate line (2.47) than below the substrate line (1.47) (Table 4). For shoots that were produced, pruning did not influence average new shoot length (Table 1). Average new shoot length (in.) on unpruned plants did not differ from lengths on plants pruned at 2 or 6 inches (Table 1). Average total shoot length did present differences among treatments. On unpruned plants, average total shoot growth from below the substrate line (13.00) exceeded shoot growth originating above the substrate line (4.67) (Table 2). For plants pruned at 2 inches, no difference occurred for shoot growth for the below (14.75) and above (12.37) substrate positions (Table 3). For plants pruned at 6 inches, average total shoot length above substrate level (15.28) was statistically different from average total shoot length below substrate level (12.73) (Table 4).
Plants that were pruned did not produce flower buds, regardless of pruning height (data not shown). Unpruned plants did occasionally produce flower buds.
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Table 2. Total number of shoots and average total shoot length produced at two positions on plants that were not pruned.z |
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Position |
Number of Shoots |
Average Total Shoot Length (in.) |
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Above substrate |
1.30 A |
4.67 B |
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Below substrate |
0.32 B |
13.00 A |
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z Means in the same column with the same letter for each variable are similar at p ≤ 0.01; n = 182. |
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Table 3. Total number of shoots and average total shoot length produced at two positions on plants that were pruned at 2 inches.z |
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Position |
Number of Shoots |
Average Total Shoot Length (in.) |
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Above substrate |
1.26 B |
12.37 A |
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Below substrate |
1.90 A |
14.75 A |
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z Means in the same column with the same letter for each variable are similar at p ≤ 0.01; n = 182. |
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Table 4. Total number of shoots and average total shoot length produced at two positions on plants that were pruned at 6 inches.z |
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Position |
Number of Shoots |
Average Total Shoot Length (in.) |
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Above substrate |
2.47 A |
15.28 A |
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Below substrate |
1.47 B |
12.73 B |
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z Means in the same column with the same letter for each variable are similar at p ≤ >0.01; n = 182. |
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Plant branch height, compactness, and uniformity can be influenced by pruning Aesculus parviflora during container production practices. Pruning at 2 or 6 inches above the substrate line increased branching and improved the quality of the plant versus those unpruned. Pruning at 2 inches above the substrate line increased the number of stems arising from the base versus pruning at 6 inches. This should benefit the appearance of plants marketed in 3- or 4-quart container sizes. Work is continuing to see if either of these pruning heights will influence plant quality when it is moved to 3-gallon or larger production sizes. By achieving better quality in plant appearance through more stem development and lower branching, Aesculus parviflora may have better sales appeal at the retail level.
Statistical analysis was completed with the assistance of Dr. John Snyder, Department of Horticulture, University of Kentucky.
Manjul Dutt and Robert L. Geneve, Department of Horticulture
Seed germination begins with the initiation of water uptake by the dry seed and ends with the protrusion of the radicle from the fully imbibed seed. Measurement of initial water uptake is usually by measuring fresh weight gain, which is laborious and requires physical handling of each seed. Such techniques require pooling of seeds to make different samples to estimate average values and submit to statistical analysis. These methods do not record growth performance and variation on an individual seed basis. Dell Aquilla et al. (2) and McCormac and Keefe (8) have described image analysis systems to monitor the imbibition in cabbage and cauliflower seeds. Such techniques, though useful, require the setup of sophisticated and expensive equipment. The computer imaging system developed by Geneve and Kester (4) uses a simple Petri dish germination system that is inexpensive and amenable to automated capture of sequential digital images in real time.
In this study, the techniques developed by Geneve and Kester (4) were used to evaluate seed dormancy release in two woody legume species with different dormancy types. Honeylocust (Gleditsia triacanthos L.) seeds have physical dormancy and require scarification to allow imbibition. The objective was to show how this computer-aided system could document initial water uptake in seeds following physical or acid scarification. Eastern redbud (Cercis canadensis L.) seeds have physiological dormancy and require chilling stratification(6). In this case, radicle growth in excised embryos is an indicator of release from dormancy following chilling. Therefore, radicle length was measured on an hourly basis in non-chilled and chilled seeds to determine specific growth rates.
Seeds of honeylocust were acid scarified for 30 or 60 minutes in concentrated H2SO4 or physically scarified by nicking the center of the seed using a file. Seeds of redbud were treated with concentrated H2SO4 for 30 minutes and stratified at 4°C for four weeks. Non-stratified seeds were acid scarified but did not receive chilling. After four weeks, embryos were surgically removed from redbud seeds.
Two honeylocust seeds or four redbud embryos were placed in 6-cm-diameter plastic Petri dishes containing one piece of transparent cellulose film (Celorey-PUT, Cydsa Monterrey, Mexico). Honeylocust seeds were surface sterilized in 10% Clorox® solution for 10 minutes and washed in distilled water before being placed in a Petri dish containing 2 ml of distilled water. Petri dishes were sealed with Parafilm® and placed on a flat-bed scanner (HP Scanjet 5370 C with transparency adapter). The scanner was controlled using a SigmaScan Pro 5.0 for Windows (SPPC Science, Chicago, Ill.) macro written in Visual Basic that allowed for timed interval scans. For this experiment, scans were taken at hourly intervals. Gray-scale images (stored as 200 dpi, TIFF files) were analyzed using another SigmaScan® macro that allowed for batch processing of the various images in a short period of time. Data were recorded for percentage increase in seed size until radicle emergence for honeylocust and radicle length (mm) for three days in excised redbud embryos.
Seeds treated with concentrated H2SO4 for 60 minutes had a faster imbibition rate compared with seeds that were acid scarified for 30 minutes or physically scarified (Figure 1). Seeds treated with concentrated H2SO4 (60 minutes) reached 50% of their final imbibed size within 11 hours after imbibition, compared to 20 hours for physically scarified seeds (significant at P 0.01). Seeds treated with concentrated H2SO4 (30 minutes) remained small and did not imbibe much water or germinate for the study period.
Figure 1. Seed area in acid vs. physically scarified honeylocust seeds.
According to Woodstock (9), hardseededness may be due to a compact arrangement of cellulose microfibrils in the cell wall, involving an irreversible change in micellar structure during maturation and dehydration of the seed. Honeylocust seeds have a palisade epidermal layer with thick-walled malpighian cells. Subsequently, 30-minute acid scarification was not enough to adequately scarify the epidermal layer leading to reduced imbibition.
There was less variation in the rate of water uptake between seeds treated with concentrated H2SO4 (60 minutes) compared to physically scarified seeds (Figure 2). This may be due to a larger and more uniform disruption of surface area cells in acid scarified seeds compared to a single wound site on the seed coat for nicking or may be due to the non-precise nature of physically nicking the seeds. However, Figure 2 does show how the imaging system can easily compute water uptake on a single seed basis for such an analysis.
Figure 2. Impact of stratification on increase in size in individual honeylocust seeds.
Baskin et al. (1) suggested that in legume seeds the lens (strophiole) is the first place on the seed coat for water entry when hard seeds become permeable under natural conditions. In contrast, for acid scarified legume seeds, Liu et al. (7) showed a general reduction in the materials covering macrosclerieds throughout the seed. Therefore, rather than a single entry point for water, it would be anticipated that acid-treated seeds would show uniform water uptake over the entire seed surface. However, when water entry was followed on an hourly basis, acid-treated honeylocust seeds showed asymmetric water uptake across the seed with more water initially entering at the chalazal and micropylar ends that produced a "dumbbell"-shaped appearance (Figure 3). This suggests that the cells in the polar regions of the seed were more susceptible to acid scarification than cells in the middle of the seed.
Figure 3. Water entry over the first 45 hours in honeylocust seeds treated with acid showing the "dumbbell" shape in partially imbibed seeds.
Physically scarified seeds showed initial water uptake at the point of nicking with water spreading from the center of the seed to the opposite ends of the seed or from one end to the other end of the seed depending on the initial nicking point. The sequential images captured by the flat-bed scanner allowed us to document the water uptake at hourly intervals and enabled us to see the position of water uptake in the honeylocust seeds as would not be possible by former techniques. One of the characteristics of seeds with non-deep or intermediate physiological dormancy is that the embryo shows increased growth potential following chilling stratification (5). Geneve (3) showed that isolated redbud embryos from chilled seeds grew faster than non-chilled embryos. However, these measurements were performed by hand and done every 24 hours. In contrast, using the computer-aided imaging system, radicle length could be measured every hour and a precise growth rate calculated with little researcher investment in time (Figure 4). As predicted, non-chilled redbud embryos took 48 hours to initiate growth and required 90 hours for radicles to reach 10 mm in length, while embryos chilled for four weeks initiated growth immediately and reached a radicle length of 10 mm in only 45 hours (Figure 4; significant at P 0.01).
Figure 4. Impact of stratification on redbud radicle length.
The two experiments described in this paper demonstrated that sequential digital images captured with the flat-bed scanner can be used for a variety of growth-related aspects of seed germination. It enabled easy identification and analysis of water entry into seeds. This technique revealed changes in seed morphology that were previously undocumented for seeds with physical dormancy. This technique can also be used for assessing seeds with other types of dormancy. Also, the use of sequential imaging holds promise for an automated system to assess seed quality in seed lots.
Sequential digital images captured with the flat-bed scanner allowed for easy identification and analysis of water entry into seeds. This technique revealed changes in seed morphology that were previously undocumented for seeds with physical dormancy. Continued research will provide additional morphological details for seeds with other types of dormancy, including physiological and morphological dormancy. The use of sequential imaging also holds promise for an automated system to assess seed quality in seed lots. This will be important for determining initial seed quality after seed harvest and for evaluating quality in stored seeds that are experiencing deterioration.
Stephen Berberich, Mark A. Williams, and Robert Geneve, Department of Horticulture
Passion flowers are members of the genus Passiflora and are among the most exotic flowers in cultivation. The Passiflora genus includes many species and hybrids with a vast diversity of color and shape of flowers and foliage (5). Although most passion flowers are easily propagated from cuttings (2), there is little information available to growers about the cultural practices necessary for successful nursery production of these vines.
The overall objective of this project is to produce tropical vines with unusual flowers for the summer garden center container market using standard outdoor nursery production (Figure 1). This will require cultural practices that maximize growth and flower production.
Figure 1. Production schedule for single-season container-grown passion flowers in Kentucky.
In the summer of 2001, a preliminary study carried out using Passiflora 'Blue Bouquet' determined that fertilizer concentration had a significant impact on shoot length. Therefore, the objective of the current study was to evaluate effect of increasing fertilizer concentrations on shoot length, flower number, and biomass in several cultivars from diverse genetic backgrounds.
Four cultivars, Passiflora 'Blue Bouquet', P. 'Amethyst', P. 'Fledermouse', and P. 'Lady Margaret' were propagated from two node cuttings taken in early March, treated with indole-3-butyric acid (IBA) (1,000 ppm in talc), and stuck in Oasis rooting cubes. Cuttings were placed in an intermittent mist bed (5 sec. every 10 min.) with bottom heat (75°F). After three weeks, cuttings were moved to 4-inch plastic containers with a peat/bark medium (Scott's Metro Mix 360) and placed in the greenhouse. The greenhouse was maintained with day/night temperatures at 78/68°F. Plants were fertilized with a 100 ppm fertilizer solution (Peter's 20-10-20) at each watering.
Plants were moved to 5-quart containers (Nursery Supplies Inc. Classic 500) in Barky Beaver (Professional Grow Mix, Moss, Tenn. 38574) southern pine bark substrate on May 15, 2002, and moved to the outdoor nursery and placed on trickle irrigation. Each container was treated with slow-release fertilizer (Osmocote 14-14-14) at 15, 20, or 25 grams per container. The plants were harvested after two months of growth in the nursery (July 15) and evaluated for number of stems, stem length, number of nodes, dry weight, and number of flowers.
All cultivars, except P. 'Fledermouse' produced greater shoot length (Figure 2) and biomass (Figure 3) with 25 compared to 15 grams of fertilizer. P. 'Amethyst' and P. 'Blue Bouquet' showed the most significant increase in shoot length (72% and 50%, respectively) and biomass (92% and 49%, respectively).
Figure 2. Passion flower total shoot length after two months treated with different levels of fertilizer (Osmocote 14-14-14).
Figure 3. Passion flower dry weight after two months treated with different levels of fertilizer (Osmocote 14-14-14).
The results for flower numbers, though, were quite different. P. 'Fledermouse' and P. 'Blue Bouquet' showed no increase in flowering at higher levels of fertilizer. However, P. 'Amethyst' showed a 93% increase in flower number when fertilizer was increased from 15 g to 25 g and P. 'Lady Margaret' showed a 15% increase in flower number with the same increase in fertilizer (Figure 4).
Figure 4. Mean number of flowers after two months treated with different levels of fertilizer (Osmocote 14-14-14).
One possible reason for these data is that, toward the end of the production cycle, the fertilizer was becoming depleted. Controlled release fertilizers such as Osmocote can increase nutrient release by as much as 30% for every 10°C increase in temperature (4). Therefore, nutrient loss can be quite severe during hot weather. During the nursery production phase, with the containers in direct sunlight, they can build up considerable heat, and the substrate temperature can rise well above ambient temperature (1). Furthermore, although the manufacturer's recommended rate of fertilizer for this size container is 14 grams, because of the high growth rate of passion flowers, a higher concentration may be necessary. Due to nutrient leaching and the effect of increased temperatures on the controlled release fertilizers, multiple applications may be necessary to prevent nutrient supply depletion as the season progresses (3).
A probable reason for the data on flower numbers is the genetic diversity of the plants studied. It is quite possible that some of these varieties will not flower early in the season regardless of cultural practices. Indeed, this is the most likely explanation for the results collected for flowering of P. 'Fledermouse' and P. 'Blue Bouquet'. Further evaluation is needed to determine is flowering is influenced by cultural practices or if flowering in these varieties is a factor of the plants' genetics.
Although P. 'Lady Margaret' had the shortest stem length of all varieties tested, it had the greatest number of flowers, and it had the highest bio-mass relative to stem length. It produced a plant with much heavier stems and leaves along with increased flowering. The increase in fertilizer concentration resulted in an increase in stem length, flower number, and bio-mass for P. 'Amethyst'. Two varieties, P. 'Fledermouse' and P. 'Blue Bouquet', both produced long stems; however, neither one had a significant number of flowers. Indeed, many of these plants had no flowers at all in the time allowed by the given production schedule. Ultimately, P. 'Lady Margaret' and P. 'Amethyst' showed the greatest promise and were most productive when grown using this production scheme.
This is the second report on studies carried out to evaluate the production of container-grown passion flowers. This study has shown that selected varieties can be successfully grown in Kentucky as a single-season crop using the production schedule presented above. Acceptable plants can be grown in the two-month production scheme in an outdoor nursery using one application of 25 grams of Osmocote 14-14-14 fertilizer. These plants have good potential as a high-value, container-produced plants for patio or garden use in a market where customers are looking for exotic, tropical vines.
Amy Fulcher, Cindy Finneseth, and Winston C. Dunwell, Departments of Horticulture and Regulatory Services
Kentucky has 2,050 (T. Pescatore, UK Poultry Extension Specialist, personal communication) poultry houses producing approximately 150 tons of animal waste per house each year (7). Poultry litter is potentially an inexpensive and readily available product for the Kentucky nursery industry. While studies have indicated that various sources and forms of composted animal waste can be used as a container substrate (1, 4, 5), literature indicates that 10 to 20% is the maximum amount by volume that can be utilized (6, 3, 2).
The objectives of this preliminary study were to determine if pelletized poultry litter could be used as a container substrate and, if so, determine the maximum amount of poultry litter that could be utilized.
Uniform rooted cuttings of Euonymus fortunei 'Emerald Gaiety', Spirea x bumalda 'Goldflame', and Euonymus alatus 'Compactus' were potted on August 9-10, 2000, into 3.8 liter (#1) containers with pine bark and 0, 5, 10, or 20% pelletized poultry litter (All Natural Organic Flower & Vegetable Fertilizer, 3-4-3, Plant Right Inc., Purdy, Mo.) by volume. The experiment was a randomized complete block design with 10 replications of each treatment. Plants were grown on a gravel pad with overhead irrigation, consistent with normal production practices at Metcalfe Landscaping and Garden Center, Madisonville, Kentucky. Plants were not topdressed with commercial fertilizer.
A visual assessment was made whether plants were alive or dead, and growth was measured on August 28, 2000. Plant quality rating criteria are shown in Table 1. Data were subjected to statistical analysis using ANOVA and mean separation.
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Table 1. Criteria used to assess plant quality of three ornamental species grown in pine bark substrate supplemented with pelletized poultry litter. |
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Rating |
Criteria |
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0 |
Dead |
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1 |
Alive, some burning or chlorosis |
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2 |
Alive, green, no new growth |
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3 |
< 1.5" of new growth |
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4 |
1.5"- 2.5" of growth |
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5 |
> 2.5" of growth |
For all species studied, addition of poultry litter did not significantly improve plant quality as compared to untreated plants (Tables 2 through 4). However, addition of poultry litter at 20% by volume significantly reduced quality for all three species, indicating that this level exceeds that which could be used in a nursery setting. For Euonymus alatus 'Compactus', substrate of 10% poultry litter significantly reduced plant quality.
|
Table 2. Plant quality evaluation of Euonymus alatus ‘Compactus’ grown in pine bark substrate with addition of 0, 5, 10, or 20 percent poultry litter by volume. |
|
|
Euonymus alatus 'Compactus' |
|
|
Poultry Litter Concentration |
Rating* |
|
0 |
2 A |
|
5 |
1.7 ± 0.48 A |
|
10 |
0.4 ± 0.52 B |
|
20 |
0 B |
|
* Means followed by the same letter are not significantly different. |
|
|
Table 3. Plant quality evaluation of Euonymus fortunei ‘Emerald Gaiety’ grown in pine bark substrate with addition of 0, 5, 10, or 20 percent poultry litter by volume. |
|
|
Euonymus fortunei 'Emerald Gaiety' |
|
|
Poultry Litter Concentration |
Rating* |
|
0 |
2.1 ± 0.32 A |
|
5 |
2.3 ± 1.25 AB |
|
10 |
1.8 ± 1.14 AB |
|
20 |
0.3 ± 0.95 B |
|
* Means followed by the same letter are not significantly different. |
|
|
Table 4. Plant quality evaluation of Spirea x bumalda 'Goldflame'’ grown in pine bark substrate with addition of 0, 5, 10, or 20 percent poultry litter by volume. |
|
|
Spirea x bumalda ‘Goldflame’ |
|
|
Poultry Litter Concentration |
Rating* |
|
0 |
4.4 ± 0.84 A |
|
5 |
4.8 ± 0.42 A |
|
10 |
3.6 ± 1.17 A |
|
20 |
0 B |
|
* Means followed by the same letter are not significantly different. |
|
Spirea x bumalda 'Goldflame' had higher plant quality than Euonymus alatus 'Compactus' under all treatment conditions.
Flies congregated and laid eggs on the bottoms of containers, creating unsavory working and retail sales conditions. In addition, a strong odor necessitated the experiment being relocated to a site further away from retail customer traffic. While this may not be a serious concern for wholesale nurseries, it may impact shipping to retail locations. Shrinkage of 25% was a concern observed in the pots containing 20% poultry litter.
An abundance of poultry litter is found throughout many states in the southeastern United States. Poultry litter is an acceptable substrate in small quantities for some nursery crops. While some crops may tolerate 10%, a maximum of 5% by volume poultry litter should be observed for certain crops. Testing for the optimum percent poultry litter for each new crop is necessary. Testing each new source of poultry litter is also advised.
The authors wish to express their appreciation to Metcalfe Landscape and Garden Center, Madisonville, Kentucky, and Plant Right Inc., Purdy, Missouri.
David W. Held and Daniel A. Potter, Department of Entomology
The Japanese beetle (JB), Popillia japonica Newman, is a voracious plant-feeding insect and a historic pest of roses (Rosa hybrida). Cultivated roses are described as "perhaps the most preferred" of all the ornamental plants (2). Beetles typically alight on blooms, due possibly to their presence on the plant, their alluring odor, or their striking colors. The characteristic top-down feeding pattern of Japanese beetles on tree hosts, like linden, suggest that these beetles may alight on rose blooms because of their presence on the plant, or plant height in general. Some rosarians have observed, in mixed plantings of hybrid tea roses, that taller plants are often attacked first before more compact flowering roses. Beetles are also attracted to a range of plant-produced volatiles, particularly those that are floral or fruity in nature. In fact, early USDA entomologists suggested that "odor is probably the most important factor in the beetle's selection of a plant" (1). Visual cues, like color, are also used in plant selection. Artificial flowers that are white and yellow are landed on by more beetles than other colors in the presence of a standard odor source.
In the present study, artificial rose flowers, or flower models, and potted hybrid tea roses with either red or yellow flowers were used to test the influence and interaction of height, fragrance, and color on selection of rose blooms by Japanese beetles. The objective of the first experiment was to determine the influence and potential interaction of bloom height, bloom color, and fragrance. In this experiment, a red and yellow flower model, of the same size, was attached to a stake placed in the center of a non-flowering potted rose. One flower of each pair was attached 23 cm above the other. There were four treatments (first being the upper, second lower, respectively); red versus yellow, yellow versus red, red baited versus yellow, yellow versus red baited. The bait was a rubber septa containing 10 ml Bulgarian rose oil concealed in the center of the flower model. This experiment was conducted in 4 h trials on two separate days. The number of beetles that landed on each flower model was recorded.
A second experiment evaluated the role of elevated plant height on recruitment of beetles. Potted flowering hybrid tea roses (Celebrity, yellow flowers) were transported to Spindletop farm in late July. Plants were paired so that each plant had the same number of blooms. One plant was then elevated 0.6 m above the ground using a plastic trash can. The second member of the pair was placed in the grass at the base of the can. The number of beetles that landed on the flowers and foliage of either plant was recorded over 4 h. The position of the plants was reversed after 2 h.
The third experiment evaluated the response of beetles to red- and yellow-flowered rose cultivars (Table 1) that varied in the strength of their fragrance, as indicated by the breeders. It is important to note that this classification of rose fragrance is subjective and variable. Nevertheless, the use of this non-quantitative variable provides a treatment structure that is amenable to further analysis if significant differences are found. This experiment was conducted during late July to early August 2002. Flowering potted plants were transported to Spindletop and placed adjacent to a soybean field where beetles were active. Plants were grouped by the number of open flowers and placed 1 m apart with 2 m between each replicate. Beetles were counted, then removed from plants over a 4-h period. Varieties tested in trial 2 are listed in Table 1. Plants in this trial were grouped by bloom numberand arranged into replicates as previously described. The number of beetles landing on these plants was recorded for 4 h.
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Table 1. Attractiveness of select rose cultivars to Japanese beetles. |
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|
|
Rose Cultivar |
Flower Color |
Fragrance Intensity |
Mean (± SEM) Number of Beetles |
|
Trial 1 |
||||
|
|
Sunbright |
Yellow |
Light |
40.3 ± 6.2 A |
|
|
Helmut Schmidt |
Yellow |
Moderate |
17.3 ± 5.3 B |
|
|
Old Smoothie |
Red |
Light |
1.3 ± 0.3 B |
|
|
Mirandy |
Red |
Heavy |
21.3 ± 2 AB |
|
Trial 2 |
||||
|
|
Sunbright |
Yellow |
Light |
25.7 ± 5.2 A |
|
|
King's Ransom |
Yellow |
Moderate |
18.3 ± 1.9 A |
|
|
Helmut Schmidt |
Yellow |
Moderate |
6 ± 1.5 B |
|
|
Old Smoothie |
Red |
Light |
1.3 ± 0.7 B |
|
|
Night ’n’ Day |
Red |
Moderate |
4.3 ± 0.3 B |
|
|
Opening Night |
Red |
Light |
0 B |
|
Within each trial, means followed by the same letter were not significantly different (Tukey's HSD, P < 0.05). |
||||
The first experiment with rose models showed that, among unscented flowers, significantly more beetles landed on yellow than red flowers. This confirms the results of previous work with flower models showing more beetles landing on yellow and white flowers. Among unbaited flowers, there was also no difference in the number of beetles landing on the upper or lower flowers for each color. When a yellow flower was paired with a baited red flower, however, the number of beetles landing on red, baited flowers was significantly more than the yellow flowers. This experiment suggests that the position of the flower on the plant is not as significant as the influence of color or odor. Likewise, the addition of fragrance to a less attractive red flower can make it significantly more attractive than a yellow flower. In general, the plants that had baited flowers had significantly more beetles than plants with unbaited flowers.
The second experiment evaluated whether Japanese beetles prefer plants based on plant height. The same experiment conducted with flowering canna, another preferred host of Japanese beetles, showed twice as many beetles on canna plants that were elevated relative to plants on the ground. For roses, there was no difference in the number of beetles between two individual roses of the same cultivar when one was 0.5 m taller. This relatively insignificant effect of height or position in these experiments suggests that observations of increased susceptibility may be attributed to qualities other than height, like fragrance intensity and bloom color.
The third experiment evaluated recruitment of beetles to various rose cultivars that differed in their bloom color (red or yellow) and fragrance intensity (light or moderate-heavy). There was a significant difference in the number of beetles recruited to the cultivars evaluated during the 4 h trials. Between two lightly fragranced cultivars, Sunbright and Old Smoothie, Sunbright had significantly more beetles than Old Smoothie in both trials (Table 1). In trial 2, no beetles landed on the red-flowered, lightly fragranced cultivar, Opening Night. Among the distinctly fragrant cultivars, the average number of beetles attracted to the plants was either the same (trials 1 and 2) or significantly greater for yellow-flowered cultivars (trial 2, King's Ransom versus Night 'n'Day). In trial 1, both of the yellow varieties were not significantly different from Mirandy, a heavily fragranced red cultivar. In trial 2, there were significantly more beetles recruited to Sunbright than to any of the red-flowered cultivars. An interesting result was the significant difference among the yellow-flowered cultivars in both trials. Helmut Schmidt is described as having a moderate sweet tea fragrance unlike the more floral scents of the other varieties.
The results of tests with flower models and cultivars stress the importance of color and fragrance over height or bloom position. For example, Old Smoothie recruited relatively few beetles, but it was the tallest cultivar (about 1.5 m tall) used in the experiment, with all other cultivars ranging from 0.9 to 1.3 m. The role of odor is important to host selection by Japanese beetles; however, these results suggest that bloom color is an important secondary factor. Plant odors traveling on the wind generally serve to direct plant feeders in the direction of a host, whereas colors or contrasts may provide a more localized signal. Despite the presence of a highly attractive yellow flower, Japanese beetles continued to follow the fragrance to the less attractive red bloom.
More than 90% of the Japanese beetles recruited to a rose land on the blooms. Since blooms appear to be the attractant, certain qualities like color and fragrance should vary the degree of susceptibility to Japanese beetles. Recruitment of beetles to selected rose cultivars and flower models in this study varied with color and fragrance intensity. For example, red-flowered cultivars appear to attract fewer beetles than yellow-flowered cultivars. Opening Night, a lightly fragranced, red-flowered cultivar did not recruit any beetles during the 4 h trial. Even though it is not resistant, cultivars like Opening Night, that have a less attractive bloom color and less fragrance appear to be visited less by Japanese beetles. Conversely, flowering roses that are visually attractive (white or yellow) or that have a more intense fragrance, even with red, appear to consistently attract more beetles. Although no rose is resistant to Japanese beetles, a possible alternative tactic is to choose cultivars with floral attributes that are less attractive to Japanese beetles.
Bear Creek Gardens
Jackson and Perkins Roses
Michael E. Rogers and Daniel A. Potter, Department of Entomology
Tiphia wasps are the dominant group of parasitoids that attack white grubs. These wasps burrow into the soil where grubs are feeding on the roots of plants, deliver a sting to temporarily paralyze a grub, and then proceed to lay an egg on its victim. Upon hatching, the wasp larva, attached to the outside of its host grub, pierces the host's integument and feeds on the body fluids leading to the weakening and eventual death of its host. The wasp larva then spins a cocoon in the soil in which it overwinters and emerges the following year as an adult wasp. Tiphia vernalis is an introduced species of wasp that attacks Japanese beetle (JB) grubs. Abundant throughout landscapes in Central Kentucky, T. vernalis is active from mid-April through the first week of June parasitizing post-overwintered third-instar JB grubs. The extent to which JB grubs are controlled by T. vernalis varies at our study sites, ranging from 20 to 50% of the grub population parasitized. One factor that may be responsible for the differences in grub parasitism rates between study sites is the early-season application of grub insecticides. Imidacloprid (Merit), a commonly used grub insecticide, has a long residual effect in the soil. Because of its long residual, imidacloprid is sometimes applied as early as mid-April or early May for preventive grub control. This early application coincides with the activity of the spring-active T. vernalis. We therefore tested the effects of an early May application of imidacloprid on parasitism of JB grubs by T. vernalis.
We examined grub parasitism rates by T. vernalis in imidacloprid-treated and untreated field plots. Twenty-four PVC rings (39.0 cm diam × 10.2 cm height), arranged in a 3 × 8 randomized block design, were driven into the rough, consisting of primarily Kentucky bluegrass, at the Pendleton County Country Club near Falmouth, Kentucky. Thirty third-instar JB grubs were placed onto the surface of each ring and allowed to burrow into the soil. After 1 h, each ring was treated with imidacloprid (Merit 75 WP) at full label rate (0.4 lb [(AI]/acre; 0.45 kg [AI]/ha), half label rate (0.2 lb [AI]/acre; 0.225 kg [AI]/ha), or no treatment. After 21 d, the turf within each ring was excavated down to 20 cm, and all grubs and Tiphia cocoons were collected and taken back to the lab where grubs were examined for the presence of Tiphia larvae or eggs. A similar experiment was conducted in the lab to examine the effects of imidacloprid on grub parasitism rates as well as mortality, fecundity, and longevity of adult T. vernalis. Ten pots of perennial ryegrass were treated with either imidacloprid (Merit 75 WP) at the full label rate (0.4 lb [AI]/acre; 0.45 kg [AI]/ha), half label rate (0.2 lb [AI]/acre; 0.225 kg [AI]/ha) or left untreated. Ten JB grubs were placed into each core, and one female T. vernalis was then confined on the turf core by inverting a 12-oz clear plastic container with the bottom removed and replaced with a fine mesh screen, over the turf core. Prior to sealing of the containers, a plastic film canister lid containing a piece of dental wick moistened with 10% sugar water solution was placed on the surface of the turf core as a food source for the adult wasp. After 10 d, each core was broken apart and the grubs within examined for Tiphia eggs or larvae. Wasps were recovered from each core to determine mortality among the treatments. Wasps still alive were then monitored to determine long-term effects of exposure to imidacloprid on wasp longevity and ability to parasitize grubs.
The effect of imidacloprid application on wasp behavior was also examined. We determined whether wasps can recognize and avoid imidacloprid treated turf and whether imidacloprid treated turf impairs the wasps' ability to locate and recognize their host grubs. Ability of wasps to avoid imidacloprid treated cores was tested using a two-pot choice test. Ten pairs of perennial ryegrass turf cores (10.5 cm diam) placed in plastic containers of similar size were infested with 10 third-instar JB grubs. One core from each pair was treated with Merit 75 WP at the full label rate, while the other core was left untreated. Each pair of cores was then placed into a large clear plastic container with another container of equal size inverted over the first. This created an enclosure from which wasps could not escape. A piece of dental wick soaked in a 10% sugar water solution was placed in the bottom of each of the enclosures as a food source for the wasps. One female wasp was then introduced into each enclosure. After 10 d, each set of cores was broken apart and grubs examined for parasitism. The number of parasitized grubs in enclosures containing two untreated cores and enclosures containing one imidacloprid treated and one untreated core was compared to determine if wasps can avoid imidacloprid treated turf and the effect that a brief exposure may have on parasitism in nearby untreated turf.
The ability of Tiphia larvae to develop on hosts in imidacloprid-treated soil was examined. Sixty cores of perennial ryegrass were infested with parasitized and non-parasitized Japanese beetle grubs. Five non-parasitized third-instar JB grubs and five third-instar JB grubs bearing an egg of T. vernalis were placed into each core. Twenty cores were treated with Merit 75 WP at the full label rate, 20 cores at half label rate, and 20 cores were not treated. All cores were irrigated with 1.5 cm of water. Five cores of each treatment were sampled at 7, 14, 21, and 28 d after grubs were placed into the cores. The number of Tiphia larvae surviving, the instar of Tiphia present on the grubs at each sample date, and survival of grubs in pots treated with label rate and half label rate imidacloprid were compared to control pots.
Significantly fewer grubs were parasitized in field plots treated with imidacloprid at label and half label rate when compared to the untreated control plots (Figure 1). Similarly, there was significantly less parasitism when wasps were confined for 10 d on pots of turf treated with imidacloprid at label and half label rate (Figure 1). Despite the reduction in grub parasitism, confinement on imidacloprid-treated cores did not affect wasp survival. Only one wasp out of 30 died after being confined for 10 d on imidacloprid-treated or untreated cores. There was no significant difference in longevity of wasps confined on imidacloprid-treated cores versus untreated turf cores. Wasps from all treatments survived an average of 17.8 d after removal from turf cores. Despite no effect on mortality, exposure to imidacloprid did have a significant effect on wasp fecundity. Wasps that were previously confined on turf cores treated with imidacloprid at label rate laid significantly fewer eggs than wasps confined on untreated turf cores during the first 16 d after confinement. This effect eventually waned, with no significant difference in fecundity near the end of the experiment.
Figure 1. (upper graph) Mean number of grubs parasitized by Tiphia vernalis in field plots treated with imidacloprid at label, half label rate, and untreated control. (lower graph) Mean number of grubs parasitized in laboratory by T. vernalis confined to pots of turf containing grubs. Each pot was treated with imidacloprid at label or half label rate or was untreated.
In two-pot choice tests, wasps parasitized fewer grubs when a turf core treated with imidacloprid was present with an untreated core than wasps confined on two untreated cores. Wasps confined with two pots of untreated turf containing grubs parasitized an average of 7.1 grubs, while wasps allowed to choose between a control and Merit-treated pot containing grubs parasitized an average of 1.7 grubs. In Y-trail choice tests, wasps chose significantly more often trails containing Merit-treated frass over empty trails. When both arms of the Y trail were provisioned with frass and the frass on one arm treated with Merit, wasps showed no significant difference in trail choice.
Wasps confined for 24 h to turf cores treated with Merit did not respond to the presence of frass from JB grubs in Y-trail choice tests, whereas wasps previously confined on untreated cores significantly chose more often the trail containing frass. Of the 20 wasps exposed to cores treated with Merit, two wasps chose the arm containing frass from P. japonica, while the rest of the wasps showed no response.
Although Merit application impaired the ability of adult Tiphia to locate and therefore parasitize host grubs, there was no detrimental effect on the developing wasp larvae. Once a grub was parasitized, survival and development time of wasp larvae on hosts in Merit-treated turf was not significantly different than that of wasp larvae developing on JB grubs in untreated turf.
The results of this study show that early spring insecticide applications for grub control can dramatically reduce the benefits that Tiphia wasps provide in controlling turf-infesting Japanese beetle grubs. The insecticide Merit reduced Japanese beetle parasitism rates by inhibiting the wasps' ability to locate their host grubs in treated soil. When there is no need to target other early-season turf pests, it is recommended that preventive grub insecticide applications be applied after Tiphia flight (after the first week of June in Kentucky) to conserve benefits provided by these naturally occurring beneficial insects in controlling white grub populations.
Jamee L. Hubbard and Daniel A. Potter, Department of Entomology
Calico scale, Eulecanium cerasorum, is a pest of a variety of woody plants in urban landscapes. Calico scale was apparently introduced into the San Francisco, California, area in the early 1900s from Asia and has since spread to Kentucky and surrounding states through the transport of infested plant material. In recent years, calico scale has reached outbreak proportions in urban areas of Central Kentucky on maples, honeylocust, sweet gum, hackberries, and many other tree species. The scale encrusts the branches and covers the leaves of trees. This pest is a phloem feeder, and in large numbers, feeding can result in branch and limb dieback. Trees may be directly killed by calico scale feeding or severely weakened, consequently succumbing to woodborer attacks, drought, or other stresses. It produces copious amounts of honeydew, which may coat automobiles and other objects under infested trees. Honeydew encourages growth of sooty mold fungus that blackens foliage and bark and may interfere with photosynthesis.
In recent years, severe outbreaks of this pest have occurred on Central Kentucky horse farms, golf courses, and street plantings. The focus of our research was to study the biology of calico scale on different hosts and to determine the most effective insecticides and application methods available for use in urban landscapes. We observed the life cycle and behavior of calico scale from late February to early October on horse farms around Lexington. Scales were noted primarily on honeylocust, Gleditsia triacanthos, hackberry, Celtis occidentalis, Norway maple, Acer platanoides, sweetgum, Liquidambar styraciflua, and sugar maple, Acer saccharum. Yellow sticky cards were placed in the canopies of four Norway maple trees and four hackberry trees to obtain crawler dispersal dates.
We conducted two experiments in 2002 with eight insecticides and three application methods to target first-instar settled crawlers (nymphs) in late spring and early summer or late third-instar settled nymphs on woody tissue in early spring. Our first experiment was designed to test the efficacy of a soil-injected systemic insecticide, applied at two different times, against adults and nymphs of calico scale. In this experiment, Merit 75 WP (imidicloprid) was injected into the soil, along the drip line, of seven sugar maple trees each on 19 December and seven additional sugar maples on 03 April 2002. Seven trees were left untreated as a control. Insecticides were applied at a rate listed to control scale insects (1 oz per inch DBH) and were based on DBH of the trees. The label recommends this insecticide be applied to the soil in the fall; however, we wanted to determine if a spring treatment would be equally effective. Treatments were evaluated 06 May and 17 June 2002 for efficacy against adults and settled crawlers, respectively. Most adults on the treated trees were situated in crotches of the lower branches and along the tree trunk; therefore, counts of adult scales were made by first measuring 3.0 m up the tree trunk from the ground and 30.5 cm laterally on the lowest four branches. Number of live adult scales was then counted and compared between treatments. Once crawlers had time to settle on leaves and feed for at least 14 d, 50 leaves were collected haphazardly from each tree canopy. Number of dead crawlers and total number of crawlers was determined by observation with a stereo microscope and percent mortality was compared.
Additionally, we tested two other systemic insecticide formulations. Four trees were injected in the root flare with Inject-a-cide B with Bidrin (dicrotophos) on 31 July 2002, 66d post-crawler hatch. Each bidrin-injected tree received one capsule (2 ml) of bidrin per 5.1 cm DBH. Another four trees were injected in the root flare with Imicide capsules, containing 4.0 ml of imidacloprid, on 9 August 2002, 75 d post-crawler hatch. Each imidacloprid-injected tree received one capsule (4.0 ml) of imidacloprid per 5.1 cm DBH. Five similar trees were left untreated. Leaves were evaluated 45 to 52 d after treatment.
A third experiment targeted pre-adult calico scale. A 2% oil mixture (Superior Miscible Spray Oil) was applied with a pressurized sprayer to the entire canopy of six hackberry trees on 6 March 2002, to target late third-instar scales on the woody tissue. Six additional trees were left untreated as a control. Additionally, individual shoots on non-treated trees were sprayed with a 2% oil mixture, 3% oil mixture, or water on 12 March 2002. Applications for this test were made with hand sprayers. All insecticidal oil solutions included Breakthru spreader/sticker added at 0.31 ml per liter solution. Treatments were evaluated 14 d after application.
Our fourth experiment targeted settled crawlers on leaves of hackberry trees. Six treatments were applied to individual hackberry shoots on a single tree. Six trees in a grove on the same site were treated. Insecticides were applied with a hand sprayer to runoff on 20 July 2002. Treatments included Talstar Lawn and Tree Flowable (bifentrhin), Orthene Turf, Tree and Ornamental Spray 97 (acephate), Sevin SL (carbaryl), an insecticidal soap (Safer Brand), and 2% insecticidal oil (Superior Miscible Spray Oil). All insecticides were applied at highest rates listed for scale insects. All insecticide solutions included Breakthru spreader/sticker as before. Additionally, a water-only treatment was included. Total and dead crawlers were counted 14 d after treatment and percent mortality was compared.
The following life cycle is based on observations made during spring-summer 2002. Calico scale completes its development in a single year. Large adult females, which are black-and-white checkered, are present on the woody tissue in the spring. In late April, the females begin to suck large amounts of sap, and they swell to about 6.0 mm long and 5.5 mm high. At this time, eggs are being produced underneath the female. Mean number of eggs found ranged from 1,401 to 3,858, with the highest number from females on honeylocust trees and the lowest number from females on Norway maple trees. Around 11 May in Central Kentucky, females begin to turn brownish and become crusty, which is followed by egg hatch later. After hatch, the first-instar nymphs, which are pinkish and are no more than 0.75 mm long, begin to crawl to the leaves. Some nymphs will be wind-blown to other trees. When the nymphs get to the leaves, they will insert their mouthparts and settle (then called settled crawlers) on the leaves to feed throughout the summer. They will become more yellowish and grow to approximately 1.0 mm in length. Mean number of crawlers emerging from females ranged from 487 to 2,835. Settled crawlers appear to molt to a second instar around mid- to late July, based on the observation of empty scale insect skins. In mid-September, the same crawlers will begin moving back to the woody tissue where they will stay for the duration of the winter and early spring. After they are settled on the woody tissue, they will molt to the third instar, which is black and has a harder waxy coating. In late winter to early spring, they start feeding on large amounts of tree sap, grow quickly, and molt to the adult stage.
The winter and spring soil injections with Merit 75 WP, targeting first-instar settled crawlers, did not yield significant control of scales. The 2% oil sprays applied in early spring of 2002, targeting late-instar nymphs, also did not yield significant control of scales. Inject-a-cide B (bidrin) trunk injections yielded moderate control with 56% crawler mortality, but percent mortality on Imicide-injected trees did not differ significantly from that on untreated controls (Figure 1).
Figure 1. Percent crawler mortality due to treatment with foliar and systemic insecticides.
We obtained good to excellent control of settled crawlers on trees where individual shoots were sprayed to runoff with various insecticides. Mortality resulting from all treatments was significant. Seven, Talstar and Orthene achieved nearly 100% control (Figure 1).
The objective of this project was to study the biology of calico scale, construct a life cycle summary, and provide management options to arborists and horse farm managers. We determined that Sevin, Orthene, and, more importantly, Talstar, a reduced-risk pyrethroid, controlled settled crawlers when applied thoroughly. Despite marginal control, Inject-a-cide B (bidrin) has potential as a low-risk insecticide treatment because the possibility of drift is eliminated with this application method. Efficacy of low-toxicity insecticidal soap, in this case, did not differ significantly from untreated shoots, whereas insecticidal oil achieved average (≅50% control) control of settled crawlers. This research will help horse farm managers, golf course superintendents, and other urban landscape managers follow the progress of scale development and determine proper timing and management practices for controls of calico scale. Additional research is currently under way to assess the natural enemy complex and determining pressures governing outbreaks in urban landscapes.
Julie Beale, Paul Bachi, and John Hartman, Department of Plant Pathology
Plant disease diagnosis is an ongoing educational and research activity of the UK Department of Plant Pathology. We maintain two branches of the Plant Disease Diagnostic Laboratory, one on the UK campus in Lexington, and one at the UK Research and Education Center in Princeton. Of the more than 4,000 plant specimens examined annually, about 40% are landscape plant specimens (1).
Making a diagnosis involves a great deal of research into the possible causes of the plant problem. Most visual diagnoses involve microscopy to determine what plant parts are affected and to identify the microbe involved. In addition, many specimens require special tests such as moist chamber incubation, culturing, enzyme-linked immunosorbent assay (ELISA), electron microscopy, nematode extraction, or soil pH and soluble salts tests. This year, the laboratory is gearing up for polymerase-chain-reaction (PCR) testing, which, although very expensive, will allow more precise and accurate diagnoses. Computer-based laboratory records are maintained to provide information used for conducting plant disease surveys, identifying new disease outbreaks, and formulating educational programs.
The 2002 growing season in Kentucky provided mostly warmer than normal temperatures and below normal rainfall. All months except May were warmer than normal, beginning with January having temperatures recorded at 7 to 10 degrees above normal. Significant freezes and frosts following warm periods were observed in late January, mid-February, early and late March, and late May. The most damaging was a March 4 freeze (4 to 6°F), occurring after bud break in Central Kentucky. Statewide, wet weather generally prevailed in March, April, and May, but dry weather was prevalent during June, July, and August. Rain was spotty, and some locales suffered severe drought, while others suffered only mild drought. Summer temperatures were well above normal in addition to being dry. Rains, heavy at times, returned in September and October.
Wet spring weather favored many foliar diseases, such as anthracnose, leaf spots, and scab. Extremely warm temperatures in April favored severe fire blight in the central and eastern portions of the state. Summer heat and drought were hard on all plants, especially those with inadequate root systems.
The following diseases of importance were observed this year:
The first step in appropriate pest management in the landscape is an accurate diagnosis of the problem. The UK Plant Disease Diagnostic Laboratory assists the landscape industry of Kentucky in this effort. To serve their clients effectively, landscape industry professionals, such as arborists, nursery operators, and landscape installation and maintenance organizations need to be aware of recent plant disease history and the implications for landscape maintenance. This report provides useful information for landscape professionals.
Jennifer Flowers, John Hartman, and Lisa Vaillancourt, Department of Plant Pathology
Sphaeropsis tip blight (formerly known as Diplodia tip blight) is a common, worldwide disease of more than 30 pine species and other conifers. Typical symptoms of S. sapinea infection include stunted shoots with necrotic, stunted needles, resinous cankers, and a general decline of the tree. These symptoms lead to significant economic losses of native and exotic pines in managed plantings. Our surveys of diseased and asymptomatic Austrian and Scots pine have revealed that latent infections of asymptomatic tissues by S. sapinea are common (1). For diagnostic and research purposes, however, culturing asymptomatic pine tissues to isolate the fungus destroys the tissue, preventing further studies of latent infections. We have developed PCR primers that are specific and sensitive for S. sapinea and Botryosphaeria obtusa (a closely related fungus rarely found on surface-disinfested, symptomless Austrian pine tissues).
Develop a nested-PCR protocol utilizing the universal ITS primers and S. sapinea-specific primers to enable future studies of latently infected shoots.
A tissue sampling regime that allowed efficient identification of latently infected shoots without destroying them was developed and results of the nested-PCR were statistically compared to the results of the culturing technique.
Based on previous studies, terminal buds and 0.6 cm diameter bark samples were chosen as the tissues that would be most likely to harbor S. sapinea and least likely to cause death of the shoot upon removal (2). The symptomless samples were surface disinfested and their DNA extracted using a CTAB method. S. sapinea DNA, if present, was amplified using the nested-PCR approaching utilizing the universal ITS primers and the S. sapinea specific primers (S.sapFOR3 and S.sapREV3). The sensitivity of the nested-PCR protocol was tested using known concentrations of purified S. sapinea DNA diluted in either noninfected Austrian pine terminal buds or bark samples.
Terminal buds and bark samples were recovered as described above from 114 symptomless shoots collected from 10 tip blight diseased Austrian pines on the University of Kentucky Lexington campus between December 2001 and March 2002. Each bud or bark sample was cut in half and surface disinfested. One half of each sample was cultured on nutrient media and the other half was used as a template for the nested-PCR. A Chi-square test was used to determine the degree of correlation between the two techniques.
With the development of a sensitive and specific PCR-based test probe as well as an appropriate sampling regime, it is possible to tell if a pine tree is infected with S. sapinea long before it ever shows symptoms. However, due to the uneven distribution of the pathogen within the tree, it would be impossible to certify a whole tree "disease-free" based on a few tissue samples. Most importantly, this probe can be used as a tool to study the fungal and plant genetics affecting the shift of the pathogen from latent to active status. By knowing the mechanism behind this shift, basic research such as this could provide a way to block the shift either by changing host plant genes or by making environmental changes that would keep the fungal genes from being expressed in the host.
John Hartman, Jennifer Flowers, Lisa Vaillancourt, Marie Schira, Jerry Hart, and Larry Hanks, Department of Plant Pathology, Physical Plant Division, and Pampered Properties Inc.
Tip blight disease, caused by the fungus Sphaeropsis sapinea, is a major problem of Austrian pine (Pinus nigra) in the landscape (1). Control by pruning or spraying is difficult and usually ineffective; most affected trees eventually die or are removed (2). Young trees that are not yet producing cones are rarely affected by tip blight. It has been suggested that a primary source of inoculum may be old infected cones and that young trees escape due to lack of locally produced infective propagules. However, we have found that the tip blight fungus is present in healthy parts of trees (found in more than 70% of symptomless shoots), or in healthy young trees (17%), living as a latent pathogen or possibly as an endophyte within the twigs (3,4). This study is intended to determine whether or not fungicide injection can prevent new infections and further spread of tip blight disease (5, 6). We hope to also determine whether or not injection of pines with fungicides can eradicate S. sapinea from within infected/infested pines and the impact of fungal eradication on disease.
Two distinct groups of UK campus Austrian pines were selected for injection treatments as described below: Experiment 1, mature diseased (1 to 50% tip blight) Austrian pines (6 replicates) and, Experiment 2, maturing disease-free Austrian pines (10 replicates). Disease symptoms were evaluated in mid- to late summer each year by estimating the percent of diseased shoot tips per tree. Diseased branches previously removed for sanitation purposes were included in the estimate. During July 2000, and again in July 2001 and July 2002, shoot, bud, and needle samples (two each) from asymptomatic and diseased shoots were collected from each treated tree and the pathogen cultured on acidified potato dextrose agar (PDA) using standard microbiological techniques. The fungal cultures were identified and confirmed microscopically following inoculation of autoclaved pine needles.
Sixteen 22-year-old diseased Austrian pines located on traffic islands on the UK Lexington campus received one of four treatments. Treatments, arranged in a randomized complete block design and replicated on four individual trees, consisted of injections at labeled rates of a) oxycarboxin (Carboject), b) debacarb (Fungisol), c) tebuconazole (Tebuject) and d) water used as controls. In 1999, treatments were made 8 May (capsules removed 12 May; water controls injected 18 May to 22 May), when the candles of the pines were partly elongated. In 2000, treatments were similarly made 6 May to 9 May (capsules removed 16 May), in 2001 on 5 May (capsules removed 17 May) and in 2002 on 29 May to 30 May and 8 June (capsules removed 22 June). Due to the demise of several of the traffic island trees in the first year, eight additional 20 year-old trees located around the perimeter of a UK campus parking lot nearby were injected in May 2000, 2001, and 2002. Trees have been grouped into randomized complete blocks, and each treatment is now replicated five or six times with each replicate being an individual tree.
From a group of 71 disease-free 13- to 14- year-old Austrian pines forming a screen planting on the UK campus, 40 trees were injected in 1999, 2000, 2001, and 2002 as described above. The experiment was designed as a randomized complete block experiment with 10 replicates.
The three fungicides, Fungisol, Tebuject, and Carboject were each tested in the laboratory and compared to water controls. Fungicides were incorporated into acidified PDA petri plates at rates ranging from 1ppm to 1,000 ppm. Plugs (4 mm diameter) of mycelium from actively growing cultures of S. sapinea were transferred to the plates. The length of fungal hyphae growth from the original plug was measured four days later. The test was replicated four times, and fungicide concentrations that just prohibited fungal growth were calculated as effective doses.
Tip blight disease ratings for both Experiment 1 and 2 are presented in Table 1. After three years of injections, trees in Experiment 1 continued to die until 2002 when no new trees died. Depending on treatment, disease ratings for these mature trees in 2002 compared to 1999 have increased 121 to 245 per cent. Disease levels appear to be stabilizing in Fungisol-treated trees where 2002 disease levels had only progressed to 121% of 1999 disease levels. Disease levels for other treatments had progressed to about double the levels seen in 1999. For Experiment 2, the younger, less-diseased trees are gradually increasing in disease levels (Table 1). In 1999, there was no noticeable disease in this plot; by 2002, disease ratings ranged from 13 to 23 percent but showed no noticeable treatment effect.
|
Table 1. Three-year disease ratings (percent blighted shoot tips) for Experiment 1, mature, diseased, Austrian pines on the UK/Lexington campus (6 replicates [4 replicates begun in 1999 and the rest in 2000]), and for Experiment 2, maturing, less-diseased Austrian pines (10 replicates). |
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| Austrian Pine Trees | Experiment 1 | Experiment 2 | |||||
|
Treatment |
Rating Year |
Range |
Average* |
Average for Original Trees |
Original Trees' Disease Percent vs. 1999 |
Range |
Average |
|
1 - Fungisol |
1999 |
1-85 |
28 |
28 |
100 |
0 |
0 |
|
2000 |
1-100 |
23 |
32 |
125 |
0-<1 |
trace |
|
|
2001 |
4-100 |
25 |
31 |
111 |
0-25 |
11 |
|
|
2002 |
5-100 |
25 |
34 |
121 |
10-40 |
19 |
|
|
2 - Tebuject |
1999 |
1-90 |
51 |
38 |
100 |
0 |
0 |
|
2000 |
1-100 |
44 |
62 |
129 |
0-<1 |
trace |
|
|
2001 |
1-100 |
51 |
70 |
184 |
5-20 |
11 |
|
|
2002 |
20-100 |
62 |
78 |
205 |
10-20 |
13 |
|
|
3 - Carboject |
1999 |
9-52 |
34 |
34 |
100 |
0 |
0 |
|
2000 |
5-70 |
31 |
49 |
144 |
0-<1 |
trace |
|
|
2001 |
5-100 |
41 |
57 |
168 |
5-40 |
12 |
|
|
2002 |
5-100 |
42 |
60 |
176 |
10-75 |
23 |
|
|
4 - Water |
1999 |
23-35 |
29 |
29 |
100 |
0 |
0 |
|
2000 |
5-60 |
33 |
46 |
159 |
0-<1 |
trace |
|
|
2001 |
5-100 |
42 |
60 |
207 |
5-30 |
13 |
|
|
2002 |
5-100 |
51 |
71 |
245 |
10-40 |
16 |
|
|
* Percent disease decreased from 1999 to 2000 because new, less-diseased
trees were added after the first year. |
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Recovery of S. sapinea from pine shoots
In Experiment 1, S. sapinea was isolated from most of the diseased and symptomless shoots from 2000-2002 (Table 2). In Experiment 2, diseased shoots often yielded significantly higher levels of the pathogen in culture than did asymptomatic shoots from the same trees (Table 2). In Experiment 2, S. sapinea was isolated from all diseased shoots, whereas the fungus was isolated from only six to eight of 10 asymptomatic shoots. In 2000, a lower proportion of Fungisol-treated trees yielded the fungus from symptomless shoots than from the other treatments. Treatment differences were not noticed in 2001 and 2002. During the injection process, it was noticed that some of the capsules soon filled with pitch from the tree. Thus, it is difficult to know whether or not all capsules were actually emptied into the injection sites.
| Table 2. Isolation of Sphaeropsis sapinea from UK Lexington campus pines: Experiment 1, mature, diseased Austrian pines (6 replicates), and Experiment 2, maturing, less-diseased Austrian pines (10 replicates) treated with fungicides via trunk injections. Percent shoots yielding S. Sapinea in culture (10 samples per tree). | |||||||||
| Austrian Pine Trees |
|
|
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| Percent Recovery of S. sapinea | Percent Recovery of S. sapinea | Proportion of Trees with S. sapinea | |||||||
| Treatment and Sample Health Status | 2000* | 2001 | 2002 | 2000 | 2001 | 2002 | 2000 | 2001 | 2002 |
| 1 - Fungisol, symptomless shoot | 55 A | 50 A | 70 a | 8 a | 17 a | 32 a | 1:10 | 6:10 | 7:10 |
| 3 - Tebuject, symptomless shoot | 40 A | 50 A | 88 A | 18 A | 30 A | 20 A | 5:10 | 5:10 | 6:10 |
| 7 - Water, symptomless shoot | 67 A | 60 A | 80 A | 23 A | 25 B | 45 AB | 5:10 | 5:10 | 7:10 |
| 5 - Carboject, symptomless shoot | 46 A | 100 A | 75 A | 25 A | 26 B | 55 AB | 7:10 | 5:10 | 8:10 |
| 4 - Tebuject, diseased shoot | 75 A | 100 A | 100 A | 69 B | 87 B | 90 BC | 8:8 | 10:10 | 10:10 |
| 8 - Water, diseased shoot | 79 A | 100 A | 100A | 75 BC | 94 B | 100 C | 6:6 | 10:10 | 10:10 |
| 2 - Fungisol, diseased shoot | 80 A | 100 A | 100 A | 79 BC | 100 B | 100C | 6:6 | 10:10 | 10:10 |
| 6 - Carboject, diseased shoot | 67 A | 100 A | 100 A | 88 C | 93 B | 110C | 6:6 | 10:10 | 10:10 |
| * Means in a column followed by the same letter are not significantly
different; Waller-Duncan K-ratio t-test (K = 100, p = 0.05). Fungisol = debacarb; Carboject = oxycarboxin; Tebuject = tebuconazole. |
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In vitro test of injected fungicides
The effective dose for complete inhibition of S. sapinea hyphal growth on PDA amended with fungicides can be determined from the data presented in Table 3.
|
Table 3. Effect of different concentrations (in parts per million) of fungicides on in vitro growth of S. sapinea (in millimeters) after 4 days growth on acidified PDA amended with fungicide. |
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|
Fungicide and Concentration |
1 ppm |
2 ppm |
3 ppm |
4 ppm |
5 ppm |
6 ppm |
1,000 ppm |
|
Tebuject |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
Fungisol |
66 |
64 |
38 |
23 |
8 |
0 |
0 |
|
Carboject |
68 |
66 |
63 |
67 |
68 |
65 |
0 |
|
Water |
64 |
63 |
69 |
65 |
64 |
67 |
65 |
|
Fungisol = debacarb; Carboject = oxycarboxin; Tebuject = tebuconazole. |
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Based on this test, Tebuject completely inhibited fungal growth at the rate of only 1ppm. The effective dose may be even lower, but this concentration was the lowest used. The effective dose of Fungisol needed to stop fungal growth occurred at 6 ppm, which is still good for most fungicidal applications. However, for Carboject, inhibition did not occur until fungicide concentrations were greater than 1,000 ppm.
After four consecutive years of trunk and buttress root injections, disease progress may have been slowed significantly by injections of Fungisol. This treatment did not prevent death of some of the trees with advanced disease symptoms early in the experiment, but it did slow down the disease in survivor trees. From the in vitro test, Fungisol was active against the fungus at low levels. It is not known why injected Tebuject was ineffective even though possessing excellent fungicidal activity in the laboratory. We were unable to determine if any of the injected fungicides actually made it into the branches of injected trees. Fungicide injections did not appear to reduce levels of latent infections of S. sapinea. None of the fungicides prevented new infections or reduced disease severity in younger trees initially having no disease. This was the final year for this four-year project.
This work demonstrates that fungicide injections are not a very effective and practical way to manage Austrian pine tip blight disease. Injection treatments neither induced tree recovery nor eradicated the fungus already existing in trees or parts of trees before symptoms developed. Thus, landscape managers wishing to slow the onset and rapid spread of tip blight in existing Austrian pines will need to rely on cultural practices such as reducing environmental stresses like drought and shade in the growing site and to practice sanitation to reduce buildup of pathogen inoculum. Knowing that this disease is difficult to control may influence landscape architects and managers in deciding whether to use Austrian pine in the landscape. Indeed, unless other efficient control measures are developed, for longevity and ease of maintenance, Austrian pines may not be a good choice for Kentucky landscapes.
John Hartman, Claudia Cotton, and Julie Beale, Department of Plant Pathology
Landscape trees have long been afflicted with leaf scorch symptoms caused by environmental factors such as root damage, road salt, and drought and by wilt diseases caused by fungi (2). The association of xylem-limited bacteria with shade tree leaf scorch symptoms was first made in 1980 (8). In 1987, the bacterium associated with leaf scorch was described as a new species, Xylella fastidiosa (13). Bacterial leaf scorch has been reported in coastal U.S. states from New York to Texas, and in Kentucky in bur, pin, red, white, and shingle oak; sycamore and London plane; sugar, silver, and red maple; American elm and sweetgum (1,3,4,5,6,10,12).
In oak, scorch symptoms first appear in late summer in individual branches where leaves show dead margins with green tissues near the main veins and leaf petiole. Often there is a fine yellow or reddish zone between brown and green tissues. Many affected leaves