Complete Genome Sequence:
Colletotrichum graminicola strains M1.001 and M5.001. Sequencing of M1.001 was funded by the USDA-NRI, and was a collaborative effort of the Broad Institute, Lisa Vaillancourt, Martin Dickman, and Mike Thon. M5.001 data were donated by Pioneer, a Dupont Company.
C. graminicola EST DATA:
2380 EST sequences generated from strain M5.001 by Pioneer, a Dupont Company, are available on NCBI GSS. The sequences were produced from mRNAs collected from mycelium and conidiating cultures.102 partial cDNA sequences encoding secreted proteins identified by yeast signal sequence trap technique (described in the article by Krijger et al., 2008) are available on the NCBI nucleotide database, Genbank accession numbers AM944392-AM944494
Krijger, J., Horbach, R., Behr, M., Schweizer, P., Deising, H., Wirsel, S. 2008. The yeast signal sequence trap identifies secreted proteins of the hemibiotrophic corn pathogen Colletotrichum graminicola. Molecular Plant Microbe Interactions 21(10): 1325-1336EST data from other Colletotrichum species
C. acutatum
1003 EST, Woobong Choi, on NCBI dbEST (germinating spores).C. gloeosporioides
1029 EST, Dov Prusky, on NCBI dbEST (fungus treated with alkaline condition).C. gloeosporioides f. sp. aeschynomene
1413 EST unigenes, Amir Sharon, on COGEMEC. trifolii
540 EST unigenes, Deborah Samac, on COGEME and on NCBI dbEST.
C. graminicola full-length and partial gene sequences on GenBank:
β-tubulin 1 (TUB1) M34491
β-tubulin 2 (TUB2) M34492
pyrimidine biosynthetic gene (orotate phosphoribosyl transferase) S47907
chitin synthase V (chsA) DQ132981
chitin synthase A (chsI) AY052545
chitin synthase B (chsIII) AY052546
chitin synthase C (chsV) AY052547
cytochrome B (partial) AY285743
superoxide dismutase (sod2) AF430836
phosphopantetheinyl transferase (PPT1) DQ028305
Elongation Factor 2 (EF2) DQ512324
Heterotrimeric G protein gene (CGG1) similar to Aspergillus nidulans FadA EU589331
BOup2* gene, similar to 3-phytase A (myo-inositol hexakisphosphate phosphohydrolase A) in SwissProt Accession Number O00107 DQ512320
BOup6* gene, similar to acid phosphatase PHO1 in SwissProt Accession Number P52291 DQ512321
BOdown1* gene, similar to Aspergillus fumigatus Af293 extracellular lipase in XP_754236 DQ512322
BOdown198* gene DQ512325
BOup 1* gene DQ512319
BOdown 2* gene DQ512323
* BO refers to “breakout zone”, “up” is up-regulated and “down” is down-regulated. These genes were differentially regulated during biotrophic growth in maize stalks, as reported in the article by Tang, W., Coughlan, S., Crane, E., Beatty, M. and Duvick, J. 2006. The application of laser microdissection to in planta gene expression profiling of the maize anthracnose stalk rot fungus Colletotrichum graminicola, Molecular Plant Microbe Interactions 19(11), 1240-1250
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Genes from other Colletotrichum species in Genbank, by category:
DIFFERENTIALLY EXPRESSED cDNA SEQUENCES: Isolated using differential hybridization techniques.
C. lagenarium
Produced during appressorial development
C. lindumuthianum
C. gloeosporioides
Hard-surface contact induced in avocado strain
Produced during appressorial development in avocado strain
Nitrogen-starvation induced in Stylosanthes strain
mRNA U94187
glutamine rich gene (U94186)
mRNA U94185
mRNA U94184
mRNA U94183
mRNA U94182
mRNA U94181
mRNA U94180
Kinases
MAP kinase from C. lagenarium
MAPKK from C. gloeosporioides
histidine kinase from C. gloeosporioides
kinase TB3 from C. trifolii
serine-threonine protein kinase from C. trifolii
serine-threonine protein kinase from C. lindemuthianum
cAMP-dependent protein kinase: regulatory subunit from C. trifolii
cAMP-dependent protein kinase: catalytic subunit from C. trifolii
Calcium signaling
calmodulin from C. trifolii
calmodulin from C. gloeosporioides
calmodulin-dependent protein kinase from C. gloeosporioides
Ras homologs
Actin
Tubulin
β-tubulin 1 from C. gloeosporioides
β-tubulin 2 from C. gloeosporioides
β-tubulin 1 from C. graminicola
β-tubulin2 from C. graminicola
Pectin lyase
C. gloeosporioides f.sp.malvae pectin lyase 1
C. gloeosporioides uncharacterized strain; pectin lyase A
Pectate lyase
C. gloeosporioides f.sp. malvae pectate lyase 1
C. gloeosporioides f.sp. malvae pectate lyase 2
C. gloeosporioides avocado strain; pectate lyase B
C. gloeosporioides uncharacterized strain; pectate lyase
C. magna pectate lyase
Endopolygalacturonase
C. gloeosporioides avocado strain; polygalacturonase
C. magna endopolygalacturonase
C. lindemuthianum endopolygalacturonase 1
C. lindemuthianum endopolygalacturonase 2
Cutinase
C. capsici papaya strain cutinase
C. gloeosporioides papaya strain; cutinase
The Sorghum bicolor EST database. Compiled by M.-M. Cordonnier-Pratt, A. Gingle, R. Dean, M. Sudman, and L.H. Pratt. This collection of more than 10,000 EST sequences is available from GenBank.
Two-week-old sorghum plants (BTX 623 cultivar) were infected with pathogen (isolate FRM42I of Colletotrichum graminicola (note, should be C. sublineolum) which is a sorghum isolate). RNA was prepared from infected leaves harvested from 45 seedlings 48 hours after inoculation. Note: young seedlings (2 weeks old) exhibit juvenile resistant reaction, which is an incompatible interaction. As they grow older (4 weeks or older), plants resume susceptiblity to anthracnose disease. The library was made from poly-A RNA in the cloning vector lambda ZAP II. Clones to be sequenced were prepared by mass excision.
