The Vaillancourt
Laboratory
Department of Plant
Pathology
University of
Kentucky
Plasmid Vector Catalog
- pGEM-3Zf+: Promega
This vector can be used as a standard
cloning vector, as templates for in vitro transcription, and
for production of circular ssDNA for sequencing due to the
presence of the origin of replication of the filamentous phage
f1. The presence of the gene encoding the lacZ a-peptide allows
recombinants to be selected by blue-white screening.
- pT7blue: Novagen
pT7blue contains the pUC19 backbone, a
T7 promoter, f1 origin of replication, and modified multiple
cloning region. The multiple cloning region contains an
EcoRV site used for blunt cloning flanked by an
NdeI site, which allows PCR fragments to be
conveniently subloned into the NdeI sites of
many pET vectors.
- pUC19
pUC19 is a small, high copy number
E.coli plasmid cloning vector that is part of a
series of related plasmids constructed by Messing and
co-workers (Yanisch-Perron et al., Gene 33, 103-119). The pUC
plasmids contain portions of pBR322 and M13mp19.
- pZL1: Life Technologies
pZL1 is an E. coli
cloning vector derived from pSPORT 1 containing a
loxP sequence installed at one of the
BspHI sites and the phage P1 incA
incompatibility locus at the other BspHI site.
All other features of pSPORT are preserved in pZL1. This vector
is excised from l
ZIPLOXTM DNA by cre-mediated recombination.
No picture available.
- pBR322
pBR322 carries genes that confer
tetracycline and ampicillin resistance. It was constructed from
several naturally occuring plasmids (Balbas et al., Gene 50:
3).
No picture available.
- pBluescript II KS+:
Stratagene
The pBluescript phagemid was derived
from pUC19. The KS designation indicates that the polylinker is
oriented so that lacZ transcription proceeds from
KpnI to SacI. The
phagemid contains an f1 origin of replication, a ColE1 origin,
the lacZ gene interupted by the polylinker region to facilitate
blue-white screening, and an ampicillin resistance
gene.
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