• UK Home
• College Home
• Site Index
• Search
• People
• Help

Department of Plant Pathology

• Vaillancourt Lab Home Page
• Introduction
• Lab Members
• Colletotrichum Genes and Genome Resources
• Protocols
• Seminars
• Publications

Fungal Genetics and Plant Pathology Research

Common Laboratory Protocols

Fungal DNA Prep

Maxi-prep
Everyday Midi-prep
QUICK Mini-prep
High-throughput DNA prep method

Fungal RNA Prep

Qiagen Method
Trizol Method
Northern Blot

DNA Quantification on the Fluorometer (pdf)

Use of the Lyophilizer (Freeze Dryer)

Bacterial colony PCR

Southern Blot

Protocol 1: Non-radioactive
Protocol II: Radioactive

Bacterial Electroporation (pdf)

Storage of Fungal Cultures

Staining Protocols

DAPI Nuclear Staining
Plasmolysis
Tissue Clearing
Viability Staining
Acridine Orange

Fungal Protoplast Prep

From Mycelium (pdf)
From Oval Spores (pdf)
From Oval Spores (Thon)

Fungal Transformation

Colletotrichum Quicker Method
Colletotrichum Slower Method (pdf)
Gibberella zeae
Agrobacterium-Mediated Transformation of Colletotrichum (pdf)

Fungal Matings

Glomerella graminicola
Gibberella zeae

Plant Growth and Inoculations

Growing Corn in the Greenhouse (pdf)
Using the Dew Chamber (pdf)
Corn Inoculations

Stalk Inoculations (pdf)
Leaf Inoculations
Serenella Sukno’s Leaf Anthracnose Assay

Media Recipes (pdf)

Biological Collections

Genomic and cDNA Libraries

Fungal Strains

Colletotrichum spp.
Fusarium graminearum
Schizophyllum commune
Diplodia sp.

Bacterial Strains

Plasmid Vectors

Plants

Return to Vaillancourt Lab Home Page