Parthasarathy
Ramaseshadri
Post-Doctoral Scholar | Palli Laboratory
Department of Entomology
S-225 Agricultural Science Center N
University of Kentucky
Lexington, KY 40546-0091
Ph: 859.257.2692
Fax: 859.323.1120
Email: parthy@uky.edu
RESUME
- Nationality: Indian
- Permanent residency in US: In process, currently on H1B
- Present Address: 1729, Nichollasville Rd. Apt#9, Lexington, KY- 40503.
- Work Address: S225, Agriculture Science Center, Entomology
University of Kentucky, Lexington, KY- 40546. - Phone: Work# (859) 257-1134, Mobile# (859) 338-3765
- Email: parthy@uky.edu
RESEARCH AND WORK EXPERIENCE
- Research Associate July 2008 – till date
Department of Entomology, University of Kentucky
Lexington, KY- 40546, USA
Project: Hormonal regulation of insect metamorphosis and reproduction - Postdoctoral Research Scholar September 2004
– June 2008
Department of Entomology, University of Kentucky
Lexington, KY- 40546, USA
Project: Molecular mechanisms of juvenile hormone action in insects - Postdoctoral Research Scholar September 2002
– August 2004
Department of Biotechnology (DBT) Fellowship
Indian Institute of Science (IISc), Bangalore- 560 012 . India
Project: Genomics Initiative at IISc: expression profiling - Research Associate November 1997 – August
1999
Project Directorate of Biological Control (ICAR)
Hebbal, Bangalore- 560 091. India.
Project: Use of entomopathogenic nematodes for the biological control of insects
EDUCATION
- PhD: Agricultural Entomology 9.8/10.00 August
1999 – August 2002
Institution : Tamil Nadu Agricultural University, India. - M.Sc.: Agricultural Entomology 9.37/10.00 January
1995 – January 1997
Institution : Annamalai University, India - B.Sc.: Agriculture 4.00/4.00 August
1990 – August 1994
Institution : Annamalai University, India
RESEARCH INTERESTS
My research interest spans from basic developmental biology of insects to applied aspects of insect pest management. Insects, the tiny tinans, are the ideal model systems to study the biology and evolution of life. Though my background is in the biological control of insects pests, I bring novel molecular approaches to evolve an effective eco-friendly pest management strategies which are indispensable in the modern scientific era. Hormones play critical roles in any animal development and misregulation of gene expression by hormones lead to serious disease conditions. Synthetic hormone analogues are widely in use for insect control, but their molecular mechanisms of action remain unknown. Understanding these mechanisms help in fighting against resistance and in evolving newer potent compounds. My research goal is to understand the basics of hormonal regulation of insect development; and long-term goal is to identify a strategy which disrupts insect development thereby, paving way for an eco-friendly battle against the harmful insect pests.
Organogenesis or tissue development is a complicated process that involves inputs like nutrition, hormones, etc. I use midgut (alimentary canal/intestine) as a model system to focus on the hormonal regulation of tissue development. Midgut has multiple roles in insect development such as digestion, absorption, protection against invading pathogens, etc. Several genes regulated by hormones plays critical role in midgut development and functioning. My research focuses on the identification and characterization of these genes. I employ molecular approaches to knock-out genes and study its importance in midgut, and in broader sense, on insect development. I use mosquitoes (vector of malaria, dengue), tobacco budworm (crop pest) and red flour beetle (storage pest) as model insect systems for these studies to widen the research horizons. Also, my study expands to characterization of intestinal stem cells which have broader clinical impacts.
TECHNICAL EXPERTISE
- 1. DNA/ RNA isolation and protein purification
- 2. cDNA synthesis and labeling
- 3. design of vectors, primers, cloning, PCR, Real time PCR
- 4. histology using microtome, cryotome
- 5. immunohistochemistry, In situ hybridization
- 6. documentation using FISH, confocal microscopy
- 7. western, Southern and northern blotting
- 8. tissue culture – transfection, plaque purification
- 9. Microarray , Proteomics
- 10. knowledge on bioinformatics
PUBLICATIONS AND AWARDS (see annexure I PDF )
- Research Articles: 31
- Papers presented in symposia/conference/colloquium: 20
- Gold medals for highest OGPA: 3
- Academic prizes: 6
- First rank certificates: 2
- Best student awards: 2
- Best paper/oral presentation awards: 3
RECENT RESEARCH PUBLICATIONS
- Parthasarathy, R., Tan, A., Sun, Z., Chen, J., Rainkin, M., and Palli, S.R. 2009. Juvenile hormone regulation of male accessory gland activity in the red flour beetle, Tribolium castaneum. Mechanisms of Development. In Press. doi:10.1016/j. mod.2009.03.005.
- Parthasarathy R. and Palli, S.R. 2009. Molecular actions of juvenile hormone analogs on the metamorphosis of the red flour beetle, Tribolium castaneum. Archives of Insect Biochemistry and Physiology. 70(1): 57-70.
- Parthasarathy, R., Tan, A., Bai, H. and Palli, S.R. 2008. bHLH-PAS family transcription factor methoprene-tolerant plays a key role in JH action in preventing the premature development of adult structures during larval-pupal metamorphosis. Mechanisms of Development. 125: 601-616.
- Parthasarathy R. and Palli, S.R. 2008. Proliferation and differentiation of intestinal stem cells during metamorphosis of the red flour beetle, Tribolium castaneum. Developmental Dynamics. 237: 893-908.
- Parthasarathy, R., Tan, A., Bai, H. and Palli, S.R. 2008. Transcription factor broad suppresses precocious development of adult structures during larval-pupal metamorphosis in the red flour beetle, Tribolium castaneum. Mechanisms of Development. 125:299-313.
- Parthasarathy, R. and Palli, S.R. 2007. Developmental and hormonal regulation of midgut remodeling in a lepidopteran insect, Heliothis virescens. Mechanisms of Development. 124. 23-34.
- Parthasarathy, R. and Palli, S.R. 2007. Stage- and cell- specific expression of ecdysone receptors and ecdysone-induced transcription factors during midgut remodeling in the yellow fever mosquito, Aedes aegypti. Journal of Insect Physiology. 53. 216-229.
- Hua, B. , Parthasarathy, R. and Palli, S.R. 2007. Identification and characterization of juvenile hormone esterase gene from the yellow fever mosquito, Aedes aegypti. Insect Biochemistry and Molecular Biology. 37:829-37.
- Singh A, Kango-Singh M, Parthasarathy R, Gopinathan K.P. 2007. Larval legs of mulberry silkworm Bombyx mori are prototypes for the adult legs. Genesis, 45:169-76.
- Wu, Y., Parthasarathy, R., Hua, B., Palli, S.R. 2006. Mechanisms of midgut remodeling: Juvenile hormone analog methoprene blocks midgut metamorphosis by modulating ecdysone action. Mechanisms of Development. 123: 530-547.
- Parthasarathy, R. and Gopinathan, K.P. 2004. Comparative analysis of the development of the mandibular salivary gland and the labial silk gland in the mulberry silkworm, Bombyx mori. Gene Expression Patterns. 5: 323-339.
MEMBERSHIP
- Entomological Society of America
RECENT ATTENDANCE IN MEETINGS
- Research paper presentation in the 54th Annual Meeting of Entomological Society of America'2006 held at Reno, NV during 16-19th Nov 2008.
- Keynote Invited Guest Lecture in the International Symposium on Insect Physiology, Biochemistry and Molecular biology held at Jinan, China during 19-22nd Sep, 2007.
- Research paper presentation in the 54th Annual Meeting of Entomological Society of America'2006 held at Indianapolis, IN during 10-13th Dec 2006.
- Research paper presentation in the 5th International Symposium on Insect Molecular Science held at Tucson, AZ during 20-24th May 2006.
- Research paper presentation in the 53rd Annual Meeting of Entomological Society of America'2005 held at Fort Lauderdale, FL during 15-18th Dec 2005.
RECENT ABSTRACTS IN INTERNATIONAL MEETINGS
- Palli, S.R., Parthasarathy, R. and Tan, A. Molecular anlaysis of ecdysone action in the red flour beetle, Tribolium castaneum. Ecdysone Workshop, Ulm, Germany, July 20-24, 2008.
- Palli S.R., Parthasarathy, R. Wu Yu and Bai, H. Moelcular analysis of midgut remodling. International symposium on Insect Midgut Biology. Guangzhou, China, April 7-11, 2008.
- Palli S.R., Parthasarathy, R. and Bai, H. Juvenile hormone analog methoprene kills mosquitoes by blocking degeneration of larval tissues. Ninth International conference on JH. York, UK, 5-10 August, 2007.
- Parthasarathy R., Wu Y. Bai, H. and Palli S.R. Recent advances in understanding the mode of action of ecdysteroid and juvenile hormone analogs. 11th IUPAC International congress of pesticide chemistry. Kobe, Japan, August, 6-11, 2006.
REFERENCES
- Dr. Subba Reddy Palli
Professor
Department of Entomology
University of Kentucky
Lexington, KY- 40546.
rpalli@uky.edu
Office: 859-257-4962 - Dr. John Obrycki
Chair, Department of Entomology
University of Kentucky
Lexington, KY - 40546.
john.obrycki@uky.edu
Office: 859-257-7450
ANNEXURE I (PDF)
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