The development of an ISCOM vaccine against EHV-1. Principal Investigator: J .A. Mumford. Co- Principal Investigators: R. F. Cook and D. Hannant. Agency: The Horserace Betting Levy Board. Period: 1985-1988. Amount: £190,000
Molecular analysis of antigenic and genetic variation in equid herpes virus type 1 (subtype 1 and 2) isolates in the United Kingdom. Principal Investigator: R. F. Cook. Agency: Equine Virology Research Foundation. Period: 1986-1988. Amount: £60,700
Regulation of EIAV Replication and Disease. Principal Investigators: R. C. Montelaro, C. J. Issel. Co-Investigator: R. F. Cook. Agency: NIH/NIAID Type R01 CA49296. Period: 1996-2001. Amount: $1,592,313
EIA Vaccine Trial. Principal Investigators: R. C. Montelaro, C. J. Issel. Co-Investigator: R. F. Cook. Agency: Intervet Corp. Period: 2000-2002. Amount: $316,000
Prospective study of progeny from inapparent carriers of EIA in a herd of wild Indian horses in Southwest Oklahoma. Principal Investigator: C. J. Issel. Co-Investigator: R. F. Cook. Agency: College of Veterinary Medicine, Oklahoma State University. Period: 1997-2003. Amount: $5000
EIAV Envelope Variation and Vaccine Efficacy. Principal Investigator: R. C. Montelaro
Co-Investigator: R. F. Cook. Agency: NIH R01 AI25850. Period: 2003 - 2008. Amount: $1,091,261.
Equine Infectious Anemia Diagnostics. Principal Investigator: C. J. Issel. Co-Investigator: R. F. Cook. Agency: Idexx Laboratories. Period: 2006-2011. Amount: $409,000.
Development of Gene Delivery Approaches to Treat Equine Respiratory Diseases. Principal Investigator: D. W. Horohov. Co-Principal Investigator: R. F. Cook. Agency: Kentucky Science & Engineering Foundation. Period: 2006. Amount: $49,573.
EIAV Envelope Variation and Vaccine Efficacy. Principal Investigator: R. C. Montelaro. Co-Principal Investigator: R. F. Cook. Agency: NIAID/NIH R01A1025850-21A2. Period: 009-2014. Amount: UK 5 year budget proposed at $1,737,500.
Molecular characterization of neurovirulent EHV-1 strains. Principal Investigator: U. B. R. Balasuriya. Co-Principal Investigator: R. F. Cook. Agency: Grayson-Jockey Club Research Foundation, Inc. Period: 2010-2012. Amount: $95,361.
Comparison of in vitro antiviral activity of herpesvirus DNA polymerase inhibitors against neuropathogenic and non-neuropathogenic strains of equine herpesvirus-1. Principal Investigator: U. B. R. Balasuriya. Co-Principal Investigator: R. F. Cook. Agency: Kentucky Horse Racing Commission. Period: 2010-2011. Amount: $40,499.
Testing the susceptibility of neuropathogenic and non-neuropathogenic equine herpesviruses-1 strains to antivirals. Principal Investigator: U. B. R. Balasuriya. Co-Principal Investigator: R. F. Cook. Agency: American Quarter Horse Foundation. Period: 2010-2011.
Amount: $39,745.
Development of a genetically defined live attenuated equine herpesvirus-1 vaccine. Principal Investigator: U. B. R. Balasuriya.
Co-Principal Investigator: R. F. Cook.
Agency: Elanco Animal Health. Period: 2012-2014.
Amount: $163,752.
Identification of genetic factors responsible for establishment of equine arteritis virus carrier state in stallions. Principal Investigator: U. B. R. Balasuriya.
Co-Principal Investigator: R. F. Cook.
Agency: USDA-AFRI.
Period: 2013–2017.
Amount: $2,925,456.
Development of Recombinant Vaccines against Porcine Reproductive and Respiratory and Syndrome (PRRS): Equine Arteritis Virus-Vectored Chimeric Vaccines against PRRS.
Principal Investigator: U. B. R. Balasuriya.
Co-Principal Investigator: R. F. Cook.
Agency: Merial. Period: 2013-2015.
Amount: $444,571.
Detection of influenza nucleoprotein antigen in nasal secretions
from horses infected with equine influenza (H3N8) viruses.
R. F. Cook, R. Sinclair and J. A. Mumford. (1988). J. Virol.
Methods 20, 1 - 12.
The characterization of neutralizing and non-neutralizing
monoclonal antibodies against equine herpes virus type 1.
R. Sinclair, R. F. Cook and J. A. Mumford (1989). J. of General
Virology 70, 455 - 459.
Protection against lethal equine herpesvirus type 1 (subtype
1) infection in hamsters by immune stimulating complexes
containing the major viral glycoproteins. R. F. Cook, T.
O'Neill, R. Sinclair, B. Sundqvist and J. A. Mumford. (1990).
Vaccine 8, 491 - 496.
Retrotransposon gene engineering. R. F. Cook, S. J. Cook
and C. P. Hodgson. (1991). Nature Biotechnology 9, 748 -
751.
Liver-specific expression of a phosphenopyruvate carboxykinase-neo
gene in genetically modified chickens. (1993). R. F. Cook,
S. J. Cook, S. Savon, M. McGrane, M. Hartitz, R. W. Hanson
and C. P. Hodgson. Poultry Science 72 : 554 - 567.
Enhanced sensitivity to neutralizing antibodies in a variant
of Equine Infectious Anemia Virus (EIAV) is linked to amino
acid substitutions in the surface unit (SU) envelope glycoprotein.
(1995). R. F. Cook, S. L. Berger, K. E. Rushlow, J. J. McManus,
S. J. Cook, S. Harrold, M. L. Raabe, R. C. Montelaro and
C. J. Issel. J. Virol. 69 : 1493 - 1499.
Replication in vitro and in vivo of an equine infectious
anemia virus (EIAV) mutant deficient in deoxyuridine triphosphate
(dUTPase) activity. (1995). D. L. Lichtenstein, K. E. Rushlow,
R. F. Cook, M. L. Raabe, C. J. Swardson, G. J. Kociba, C.
J. Issel, and R. C. Montelaro. J. Virol. 69 : 2881 - 2888.
Development and characterization of an in vivo pathogenic
molecular clone of equine infectious anemia virus. (1998).
R. F. Cook, C. Leroux, S. J. Cook, S. L. Berger, D. L. Lichtenstein,
N. N. Ghabrial, R. C. Montelaro and C. J. Issel. J. Virol.
72 (2) : 1383-1393.
Immunization with a recombinant envelope protein (rgp90)
of EIAV produces a spectrum of vaccine efficacy ranging from
lack of clinical disease to severe enhancement. (1998) M.
Raabe, C. J. Issel, S. J. Cook, R. F. Cook, B. Woodson and
R. C. Montelaro. Virology 245 : 151-162.
Effects of long terminal repeat sequence variation on equine
infectious anemia virus replication in vitro and in vivo.
(1999). D. L. Lichtenstein, J. K. Craigo, C. Leroux, K. E.
Rushlow, R. F. Cook, S. J. Cook, C. J. Issel and R. C. Montelaro.
Virology 263 : 408-417
Tissue sites of persistent and active replication of equine
infectious anemia virus during acute and asymptomatic infection
in experimentally infected equids.(2000) S. M. Harrold, S.
J Cook, R. F. Cook, K. E. Rushlow, C. J. Issel and R. C.
Montelaro. J. Virol 74 (7) : 3112-3121
Differential responses of Equus caballus and Equus asinus
to infection with two pathogenic strains of equine infectious
anemia virus. S.J. Cook, R.F. Cook, R.C. Montelaro and C.J.
Issel (2001). Veterinary Microbiology, 79, (2):93-109
Multiple RNA splicing and the presence of cryptic RNA splice
donor and acceptor sites may contribute to low expression
levels and poor immunogenicity of potential DNA vaccines
containing the env gene of equine infectious anemia virus
(EIAV). W. Zhou, R. F. Cook, S. J. Cook, S. A. Hammond, K.
Rushlow, N. N. Ghabrial, S. L. Berger, R. C. Montelaro, and
C. J. Issel, (2002). Veterinary Microbiology, 88 : 127-151.
Development of a multiplex real-time reverse transcriptase-polymerase
chain reaction for equine infectious anemia virus (EIAV).
R. F. Cook, S. J. Cook, F. Li, R. C. Montelaro and C. J.
Issel, (2002). Journal of Virological Methods, 105 : 171-179.
Enhancement of equine infectious anemia virus (EIAV) virulence
by identification and removal of sub-optimal nucleotides.
(2003). R. F. Cook, S. J. Cook, S. l. Berger, C. Leroux,
N. N. Ghabrial, M. Gantz, P. S. Bolin, M. R. Mousel, R. C.
Montelaro and C. J. Issel. Virology, 313 : 588-603.
Genetic immunization with codon-optimized equine infectious
anemia virus (EIAV) surface unit (SU) envelope sequences
stimulates immune responses in ponies. (2005). R. F Cook,
S. J. Cook, P. S. Bolin, L. J. Howe, R. C. Montelaro and
C. J. Issel. Veterinary Microbiology, 108: 23-37.
Evidence of an oscillating peripheral clock in an equine
fibroblast cell line and adipose tissue, but not in peripheral
blood. (2006) B. A. Murphy, M. M. Vick,
D. R. Session, R. F. Cook, B. P. Fitzgerald. Journal of
Comparative Physiology A. 192: 743-751.
Acute inflammation transiently synchronizes clock gene expression
in equine peripheral blood. (2007). B. A. Murphy, M. M. Vick,
D. R. Sessions, R. F. Cook and B. P. Fizgerald.
Brain Behavior Immunity. 21: 467-476.
Relationship between inflammatory cytokines, obesity
and insulin sensitivity in the horse. (2007). M.M. Vick,
A.A. Adams, B.A. Murphy, D.R. Sessions, D.W. Horohov, R.F.
Cook, B.J. Shelton and B.P. Fitzgerald. Journal
of Animal Science, 85:1144-1155.
Real-time quantitative RT-PCR and PCR assays for
a novel European field isolate of equine infectious anaemia
virus based on sequence determination of the gag gene. (2007).
M. Quinlivan, R.F. Cook, and A. Cullinane. Veterinary Record
160:611-618.
Systemic inflammation influences insulin resistance in the horse and increases inflammatory cytokine expression in equine adipose tissue. (2008). M.M. Vick, B.A. Murphy, D.R. Sessions, S.E. Reedy, E.L. Kennedy, D.W. Horohov, R.F. Cook, and B.P. Fitzgerald. Amer. J.of Vet. Res. 69:130-139.
Equine neutrophils respond to PGE2 by activating expression of core circadian clock genes. (2008). B.R. Murphy, C. Merant, M.M. Vick, R.F. Cook, S.A. Brooks, D.W. Horohov and B.P. Fitzgerald. The Open Veterinary Science Journal. 2:96-103.
Full-length and internally deleted forms of interleukin-7 are present in horse (Equus caballus) lymph node tissue. (2008). R.F. Cook, S.J. Cook, D.L. Even, C. Schaffer and C.J. Issel. Veterinary Immunology and Immunopathology. 125:126-134.
Equine neutrophils respond to PGE2 by activating expression of core circadian clock genes. (2008). B. R. Murphy, C. Merant, M. M. Vick, R. F. Cook, S. A. Brooks, D. W. Horohov and B. P. Fitzgerald. The Open Veterinary Science Journal. 2: 96-103.
Equine infectious anaemia. (2009). R. F. Cook*, S. J. Cook and C. J. Issel. Equine Veterinary Education Manual: Infectious Diseases of the Horse Ed T. S. Mair and R. E. Hutchinson 56-71.
Approccio biomolecolare alla diagnosi di anemia infettiva equine. (2009). F. Passamonti, K. Cappelli, S. Capomaccio, M. Felicetti, M. L. Marenzoni, G. Coppola, A. Testa, R. F. Cook, A. Verini Supplizi and M. Coletti. Ippologia (1120-5776), 4, 20, 27-32
The increased prevalence of neuropathogenic strains of EHV-1 in equine abortions. (2010). K. L. Smith, G. P. Allen, A. J. Branscum, R. F. Cook, M. L. Vickers, P. J. Timoney, and U. B. R. Balasuriya. Veterinary Microbiology. 141: 5-11.
Complex Interactions between the Major and Minor Envelope Proteins of Equine Arteritis Virus Determine its Tropism for Equine CD3+ T Lymphocytes and CD14+ Monocytes. (2010). Y. Y. Go, J. Zhang, P. J. Timoney, R. F. Cook, D. W. Horohov, and U. B. R. Balasuriya. J. Virol. 84: 4898-4911.
Relationship between equine herpesvirus-1 myeloencephalopathy and viral genotype. (2010). S. Pronost,R. F. Cook, G. Fortier, P. J. Timoney and U. B. R. Balasuriya. Equine Veterinary Journal 42:672-674.
Molecular detection, epidemiology and genetic characterization of novel European field isolates of equine infectious anemia virus. (2011). K. Cappelli, S. Capomaccio, R. F. Cook, M. Felicetti, M. L. Marenzoni, G. Coppola, A. Verini-Supplizi, M. Coletti and F. Passamonti. J. Clin. Micro. 49:27-33.
Isolation and characterization of β-haemolytic-Streptococci from endometritis in mares. (2011). P. Casagrande Proietti, A. Bietta, G. Coppola, M. Felicetti, R. F. Cook, M. Coletti, M. L. Marenzoni and F. Passamonti. Veterinary Microbiology 152:126-130.
Assessment of correlation between in vitro CD3+ T cell susceptibility to EAV infection and clinical outcome following experimental infection. (2012) Y. Y. Go, R. F. Cook, J. Q. Fulgencio, J. R. Campos, P Henney, P. J. Timoney and U. B. R. Balasuriya. Veterinary Microbiology 157: 220-225.
Detection, Molecular Characterization and Phylogenetic Analysis of Full-Length Equine Infectious Anemia (EIAV) Gag Genes isolated from Shackleford Banks Wild Horses. (2012) S. Capomaccio, Z. A. Willand, S. J. Cook, C. J. Issel, E. M. Santos, J. K. P. Reis and R. F. Cook.* Veterinary Microbiology 157: 320-332.
Geographic structuring of global EIAV isolates: a single origin for New World strains? (2012). S. Capomaccio, K. Cappelli, R. F. Cook, F. Nardi, R. Gifford, M. L. Marenzoni, and F. Passamonti. Virus Research 163: 656-659.
A New Allelic Discrimination Real-Time PCR Assay for Detection and Differentiation of A2254 and G2254 Strains of EHV-1. (2012). K. L. Smith, Y. Li, P. Breheny, R. F. Cook, P. J. Henney, S. Sells, S. Pronost, Z. Lu, B. M. Crossley, P. J. Timoney, and U. B. R. Balasuriya. Journal of Clinical Microbiology 50: 3138-3147.
Development of a nested PCR assay to detect equine infectious anemia proviral DNA from peripheral blood of naturally infected horses. (2012) J.-B. Dong, W. Zhua, R. F. Cook, Y. Goto, Y. Horii and T. Haga. Archives of Virology (in press)
The determination of in vivo envelope-specific cell-mediated immune responses in equine infectious anemia virus-infected ponies. (2012). C. Liu, R. F. Cook, S. J. Cook, J. K. Craigo, D. L. Even, C. J. Issel, R. C. Montelaro and D. W. Horohov. Veterinary Immunology and Immunopathology (in press).
Challenges and proposed solutions for more accurate serological diagnosis of equine infectious anaemia. (2012) C. J. Issel, M. T. Scicluna, S. J. Cook, R. F. Cook, A. Caprioli, I. Ricci, F. Rosone, J. K. Craigo, R. C. Montelaro and G. L. Autorino. Veterinary Record. (in press)
Identification of a novel equine infectious anemia virus (EIAV) field strain isolated from feral horses in Southern Japan. (2012). J-B. Dong, W. Zhu, R. F. Cook, Y. Goto, H. Horii and T. Haga. J. Gen. Virol. 2012 [Epub 2012 Oct 24].
Genetic characterisation by composite sequence analysis of a new pathogenic field strain of equine infectious anaemia virus (EIAV) from the 2006 outbreak in Ireland outbreak in Ireland. (2012). M. Quinlivan, F. Cook, R. Kenna, S. Callinan and A. Cullinane. J. Gen Virol 2012 Nov 21. [Epub ahead of print].
