CURRICULUM VITAE

James N. MacLeod, VMD, PhD
jnmacleod@uky.edu
(859) 257-4757 ext 81099

Education

1980; University of Delaware, College of Agricultural Sciences, B.S., magna cum laude, with distinction.

1984; University of Pennsylvania, School of Veterinary Medicine, V.M.D.

1990; University of Pennsylvania, Faculty of Arts and Sciences, Ph.D.

1992; University of Pennsylvania, School of Medicine, Endocrinology & Genetics Fellowship.

Current Academic Appointments:

2003-present - John S. and Elizabeth A. Knight Chair / Professor of Veterinary Science, Maxwell H. Gluck Equine Research Center, University of Kentucky, Lexington, Kentucky.

2007-present - Professor of Orthopaedic Surgery, College of Medicine, University of Kentucky, Lexington, Kentucky.

2008-present - Equine Initiative Director, College of Agriculture, University of Kentucky, Lexington, Kentucky.

Previous Academic Positions:

1988-1992 - Research Fellow, University of Pennsylvania, School of Medicine and the Howard Hughes Medical Institute, Philadelphia, Pennsylvania.

1992-1999 - Assistant Professor of Molecular Genetics, James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York.

1999-2003 - Associate Professor of Molecular Genetics, James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York.

Selected Academic Awards:

1984 - Student Chapter of the American Veterinary Medical Association Prize

1986 - Phi Zeta Research Award

1992 - Dorothy Donnelley Faculty Development Award

1993 - Marilyn M. Simpson Career Development Award

1998 - Pfizer Award for Research Excellence

2008 - Dickson Professor of Equine Science and Management

Patents:

Mobile Swivel and Tether Device for Attachment to Laboratory Animals, Patent No. 4,756,770, issued July 12, 1988, assigned to the University of Pennsylvania.

Canine Erythropoietin Gene and Recombinant Protein, No. 6,696,411, issued February 24, 2004, assigned to Cornell University.

Selected Grant History:

1995-1998 - Morris Animal Foundation. Clinical Efficacy and Safety of Recombinant Canine Erythropoietin, Principal Investigator, Total direct support: $79,796.

1997-2000 - Arthritis Foundation. Cartilage-Specific Fibronectin Isoform and Osteoarthritis,  Co-Principal Investigator (Nancy Burton-Wurster, Co-PI), Total direct support: $200,149.

1997-2002 - Department of Health and Human Services - Public Health Service, National Institutes of Health  RO1 AR44340. Cartilage-Specific Fibronectin Isoform,  Principal Investigator, Total direct support: $871,506.

2001-2004 - Arthritis Foundation. Cartilage-Specific Fibronectin Isoform and Osteoarthritis, Principal Investigator (Nancy Burton-Wurster, Co-PI), Total direct support: $250,000.

2003-2006 - Department of Health and Human Services - Public Health Service, National Institutes of Health, T32 RR018269. Veterinary Student Training in Biomedical Research,  Program Director (Transferred to Mark Roberson), Total direct support: $324,740.

2006-2008 - Morris Animal Foundation.  Articular Cartilage Maturation and Repair, Fellowship sponsor for Michael Mienaltowski, Total direct support: $70,000.

2007-2011 - Morris Animal Foundation.  Program in Equine Medical Genetics, Co‑Principal Investigator (James Mickelson, Program Director), Total direct support: $33,000.

2008-2009 - University of Kentucky Health Care – Department of Orthopaedic Surgery, Metamorphosis of the Salamander as a Model of Postnatal Maturation of Articular Cartilge in Mammals.  Co-Principal Investigator (Christian Lattermann, Co-PI), Total direct support: $19,500.

Selected Publications:

MacLeod J.N., Sorensen, M.P., Shapiro, B.H. 1987. Strain-independent elevation of hepatic monooxygenase enzymes in female mice. Xenobiotica 17:1095-1102.

MacLeod, J.N., Shapiro, B.H. 1988. Repetitive blood sampling in unrestrained and unstressed mice using a chronic indwelling right atrial catheterization apparatus. Laboratory Animal Science 38:603-608.

MacLeod, J.N., Shapiro, B.H. 1989. Growth hormone regulation of hepatic drug metabolizing enzymes in the mouse. Biochemical Pharmacology 38:1673 1677.

MacLeod, J.N., Worsley, I., Ray, J., Friesen, H.G., Liebhaber, S.A., Cooke, N.E. 1991. Human growth hormone-variant is a biologically active somatogen and lactogen. Endocrinology 128:1298-1302.

MacLeod, J.N., Liebhaber, S.A., MacGillivray, M.H., Cooke, N.E. 1991. Identification of a splice site mutation in the human growth hormone variant gene. Am. J. Hum. Genet. 48:1168-1174.

MacLeod, J.N., Pampori, N.A., Shapiro, B.H. 1991. Sex differences in the ultradian pattern of plasma growth hormone concentrations in mice. J. Endocrinol. 131:395-399.

Urbanek, M., MacLeod, J.N., Cooke, N.E., Liebhaber, S.A. 1992. Expression of a human growth hormone receptor isoform is predicted by tissue specific alternative splicing of exon 3 of the human growth hormone receptor gene transcript. Mol. Endocrinol. 6:279-287.

MacLeod, J.N., Lee, A.K., Liebhaber, S.A., Cooke, N.E. 1992. Developmental control and alternative splicing of the placentally expressed transcripts from the human growth hormone gene cluster. J. Biol. Chem. 267:14219-14226.

MacLeod, J.N., Burton-Wurster, N., Gu, D.N., Lust, G. 1996. Fibronectin mRNA splice variant in articular cartilage lacks basesencoding the V, III-15, and I-10 proteins segments. J. Biol. Chem. 271:18954-18960.

Burton-Wurster, N., Lust, G., MacLeod, J.N. 1997. Cartilage fibronectin isoforms: in search of functions for a special population of matrix glycoproteins. Matrix Biol. 15:441-454.

Stewart, M.C., Farnum, C.E., MacLeod, J.N. 1997. Expression of p21CIP1/WAF1 in chondrocytes. Calcif. Tissue Int. 61:199-204.

MacLeod, J.N., Fubini, S.L., Gu, D.N., Tetreault, J.W., Todhunter, R.J. 1998. Effect of synovitis and corticosteroids on transcription of cartilage matrix proteins. Am. J. Vet. Res. 59:1021-1026.

Burton-Wurster, N., Borden, C., Lust, G., MacLeod, J.N. 1998. Expression of the (V+C)–fibronectin isoform is tightly linked to the presence of a cartilaginous matrix. Matrix Biol. 17:193-203.

MacLeod, J.N., Tetreault, J.W., Lorschy, K.S., Gu, D.N. 1998. Expression and bioactivity of canine erythropoietin. Am. J. Vet. Res. 59:1144-1148.

Randolph, J.F., Stokol, T., Scarlett, J.M., MacLeod, J.N. 1999. Comparison of the biologic activity and safety of recombinant canine erythropoietin to recombinant human erythropoietin in normal Beagle dogs. Am. J. Vet. Res. 60:636-642.

Burton-Wurster, N., Gendelman, R., Chen, H., Gu, D.N., Tetreault, J.W., Lust, G., Schwarzbauer, J.E., MacLeod, J.N. 1999. The cartilage-specific (V+C)– fibronectin isoform exist primarily in homodimeric and monomeric configurations. Biochemical J. 341:555-561.

Stewart, M.C., Saunders, K.M., Burton-Wurster, N., MacLeod, J.N. 2000. Phenotypic stability of articular chondrocytes in vitro: the effects of culture models, BMP-2 and serum supplementation. J. Bone Min. Res. 15:166-174.

Fubini, S.L., Todhunter, R.J., Burton-Wurster, N., Vernier-Singer, M., MacLeod, J.N. 2001. Corticosteroids alter the differentiaed phenotype of articular chondrocytes. J. Orthop. Res. 19:688-695.

Chen, H., Gu, D.-N., Burton-Wurster, N., MacLeod, J.N. 2002. Absence of the I-10 protein segment mediates restricted dimerization of the cartilage-specific fibronectin isoform. J. Biol. Chem. 277:20095-20103.

Burton-Wurster, N., Chen, H., Gendelman, R., Jackson, M.L., Gagliardo, L.F., Gu, D.N., Zelko, R.R., Lust, G, MacLeod, J.N. 2002. Specific immunological detection of the (V+C)– fibronectin isoform. Matrix Biology, 21:393-398.

Gendelman, R., Burton-Wurster, N., MacLeod, J.N., Lust, G. 2003. The cartilage-specific fibronectin isoform has a high affinity binding site for the small proteoglycan decorin. Journal of Biological Chemistry, 278:11175-11181.

Randolph, J.F., Scarlett, J.M., Stokol, T., MacLeod, J.N. 2004. Clinical efficacy and safety of recombinant canine erythropoietin in dogs with anemia of chronic renal failure and dogs with recombinant human erythropoietin-induced red cell aplasia. Journal of Veterinary Internal Medicine, 18:81-91.

Stewart, M.C., Kadlcek, R.M., Robbins, P., MacLeod, J.N., Ballock, T.R. 2004. Expression and activity of the CDK inhibitor p57Kip2in chondrocytes undergoing hypertrophic differentiation. Journal of Bone and Mineral Research, 19:123-132.

Randolph, J.F., Scarlett, J.M., Stokol, T., Saunders, K.M., MacLeod, J.N. 2004. Expression, bioactivity, and clinical assessment of recombinant feline erythropoietin. American Journal of Veterinary Research, 65:1355-1366.

Steffey, M.A., Miura, N., Todhunter, R.J., Nykamp, S.G., Freeman, K.P., Scarpino, V., Vernier-Singer, M.A., Erb, H.N., MacLeod, J.N., Lust, G., Burton-Wurster, N. 2004. The potential and limitations of cartilage-specific (V+C)– fibronectin and cartilage oligomeric matrix protein as osteoarthritis biomarkers in canine synovial fluid, Osteoarthritis and Cartilage, 12:818-825.

Walker, M. C., Mandell, T. C., Crawford,  P. C., Simon, G. G., Cahill, K. S., Fernandes, P. J., MacLeod, J. N., Byrne, B. J., Levy, J. K. 2005. Expression of erythropoietin in cats treated with a recombinant adeno-associated viral vector.  American Journal of Veterinary Research, 66:450-456.

Coleman, S. J., Gong, G., Gaile, D. P., Chowdhary, B. P., Bailey, E., Liu, L., MacLeod, J. N. 2007. Evaluation of COMPASS as a comparative mapping tool for ESTs using horse radiation hybrid maps.  Animal Genetics, 38:294-302.

Huang L, Zhu W, Saunders CP, MacLeod JN, Zhou M, Stromberg AJ, Bathke A.  2008. A novel application of quantile regression for identification of cartilage biomarkers in equine microarray data. BMC Bioinformatics, 9:300.

Raudsepp T, Gustafson-Seabury A, Durkin K, Wagner ML, Goh G, Seabury CM, Brinkmeyer-Langford C, Lee E-J, Agarwala R, Stallknecht-Rice E, Schäffer AA, Tozaki T, Yasue H, Penedo MC, Lyons LA, Khazanehdari KA, Binns MM, MacLeod JN, Distl O, Guérin G, Leeb T, Mickelson JR, Chowdhary BP. 2008. A 4103 marker integrated map of the horse genome. Cytogenetic and Genome Research, 122:28-36.

Mienaltowski MJ, Huang L, Stromberg AJ, MacLeod JN.  2008. Differential gene expression associated with postnatal articular cartilage maturation.  BMC Musculoskeletal Disorders, 9:149.

Haneda S, Nagaoka K, Nambo Y, Kikuchi M, Nakano Y, Matsui M, Miyake Y, MacLeod JN, Imakawa K.  Expression of interleukin-1 receptor antagonist (IL-1RN) in the equine endometrium is regulated by a steroid hormone derived from the conceptus.  Domestic Animal Endocrinology, accepted.