Joseph Chappell

Faculty

University of Kentucky, PSS
Plant Science Building
1405 Veterans Drive (Office:301B)
Lexington, KY 40546-0312
(859) 218-0775
(859) 257-7125
chappell@uky.edu

This person page last updated on 2012-05-17

Classes

ABT120 - Genetics and Society; Credits - 3
This course is designed for science and non-science majors, giving students an understanding of how genetics influences and impacts our social fabric on a daily basis, and equipping students with a sufficient understanding to participate in the policy debates that are impacting our lives. The course will introduce students to the basic concepts of genetics and to the modern methodologies of molecular genetics. The course will also educate students in the process of scientific discovery and empower students with the knowledge and critical thinking skills necessary to evaluate the present and future impact of genetics on society. While the course is intended for first semester freshmen, students at all class standings are welcome to enroll..
Instructors: Joseph Chappell

Pls601 - Special Topics in Molecular and Cellular Genetics; Credits - 1
Each semester five distinguished scientists visit the UK campus to deliver a series of three formal lectures each and participate in numerous informal contacts with graduate students. The emphasis is on the presentation of the most current advances (often unpublished) in selected topics in molecular and cellular genetics. May be repeated to a maximum of six credits. (Same as BCH/BIO/MI/PPA 601.) .
Instructors: Joseph Chappell

Research/Teaching Publications

Refereed Journal Articles

Cai, B., B. Siminszky, J. Chappell, R. E. Dewey, and L. P. Bush. 2012. Enantioselective demethylation of nicotine as a mechanism for variable nornicotine composition in tobacco leaf. The Journal of Biological Chemistry 287(51): 42804–42811. Published online: doi: 10.1074/jbc.M112.413807. (Bin Cai was a PhD student in Plant Physiology)
Authors in PSS Dept.: Joseph Chappell Lowell Bush Bin Cai

Niehaus, T., S. Kinison, S. Okada, Y. Yeo, S. Bell, P. Cui, T. Devarenne, and J. Chappell. 2012. Functional identification of triterpene methyltransferases from Botryococcus braunii race B. The Journal of Biological Chemistry 287: 8163-8173. (Thomas Niehaus was a PhD student in Plant Physiology)
Authors in PSS Dept.: Joseph Chappell Stephen Bell Scott Kinison

Niehaus, T.D., S. Okada, T.P. Devarenne, D.S. Watt, V. Sviripa, and J. Chappell. 2011. Identification of unique mechanisms fortriterpene biosynthesis in Botryococcus braunii. Proceedings of the National Academy of Sciences of the United States of America 108:12260-12265.

Faraldos, J.A., S. Wu, J. Chappell, and R.M. Coates. 2010. Doubly deuterium-labeled patchouli alcohol from cyclization of singly labeled [2-(2)h(1)]farnesyl diphosphate catalyzed by recombinant patchoulol synthase. Journal of the American Chemical Society 132:2998-3008.

Weiss, T.L., J.S. Johnston, K. Fujisawa, K. Sumimoto, S. Okada, J. Chappell, and T.P. Devarenne. 2010. Phylogenetic placement, genome size, and GC-content of the liquid hydrocarbon producing green microalga Botryococcus braunii var. Berkeley (Showa) (Chlorophyta). Journal of Phycology 46:634-540.

Albuquerque, R.J., T. Hayashi, W.G. Cho, M.E. Kleinman, S. Dridi, A. Takeda, J.Z. Baffi, K. Yamada, H. Kaneko, M.G. Green, J. Chappell, J. Wilting, H.A. Weich, S. Yamagami, S.Amano, N. Mizuki, J.S. Alexander, M.L. Peterson, R.A. Brekken, M. Hirashima, S. Capoor, T. Usui, B.K. Ambati, and J. Ambati. 2009. Alternatively spliced vascular endothelial growth factor receptor-2 is an essential endogenous inhibitor of lymphatic vessel growth. Nature Medicine 15:1023-30.

Faraldos, J.A., S. Wu, J. Chappell, and R.M. Coates. 2007. Conformational analysis of (+)-germacrene A by variable-temperature NMR and NOE spectroscopy. Tetrahedron 63:7733-7742.

Takahashi, S., Y.S. Yeo, B.T. Greenhagen, T. McMullin, L. Song, J. Maurina-Brunker, R. Rosson, J.P. Noel, and J. Chappell. 2007. Metabolic engineering of sesquiterpene metabolism in yeast. Biotechnology and Bioengineering 97:170-181.

Takahashi, S., Y.S. Yeo, Y.X. Zhao, P.E. O’Maille, B.T. Greenhagen, J.P. Noel, R.M. Coates, and J. Chappell. 2007. Functional characterization of premnaspirodiene oxygenase, a cytochrome P450 catalyzing regio- and stereo-specific hydroxylations of diverse sesquiterpene substrates. Journal of Biological Chemistry 282:31744-31754.

Xu, D.M., Y.X. Shen, J. Chappell, M.W. Cui, and M. Nielsen. 2007. Biochemical and molecular characterizations of nicotine demethylase in tobacco. Physiologia Plantarum 129:307-319.

Deguerry, F., L. Pastore, S. Wu, J. Chappell, A. Clark, and M. Schalk. 2006. The diverse sesquiterpene profile of Patchouli, Pogostemon cablin, is correlated with a limited number of sesquiterpene synthases. Arch Biochem. Biophys. 454: 123-136.

Greenhagen, B.T., P.E. O’Maille, J.P. Noel and J. Chappell. 2006. Identifying and manipulating structural determinates linking catalytic specificities in terpene synthases. Proc. Natl. Acad. Sci. 103:9826-9831.

O’Maille, P.E., J. Chappell, and J.P. Noel. 2006. Biosynthetic potential of sesquiterpene synthases: alternative products of tobacco 5-epi-aristolochene synthase. Arch. Biochem. Biophys. 448:73-82.

Schenk, D.J., C.M. Starks, K.R. Manna, J. Chappell, J.P. Noel and R.M. Coates. 2006. Stereochemistry and deuterium isotope effects associated with the cyclization-rearrangements catalyzed by tobacco epiaristolochene and hyoscyamus premnaspirodiene synthases, and the chimeric CH4 hybrid cyclase. Arch. Biochem. Biophys. 448:31-44.

Takahashi, S., Y. Yeo, B.T. Greenhagen, L. Song, J. Maurina-Brunker, R. Rosson, J.P. Noel and J Chappell. 2006. Metabolic engineering of sesquiterpene metabolism in yeast. Biotech. Bioeng. (in press, DOI 10.1002/bit. 21216).

Wu, S., M. Schalk, A. Clark, R.B. Miles, R.M. Coates and J. Chappell. 2006. Redirection of cytosolic or plastidic isoprenoid precursors elevates terpene production in plants. Nature Biotech. (in press).

Xu, D., Y. Shen, J. Chappell, M. Cui and M. Nielsen. 2006. Biochemical and molecular characterization of nicotine demethylase in tobacco. Physiologia Plantarum (in press).

Takahashi, S., Y. Zhao, P.E. O’Maille, B.T. Greenhagen, J.P. Noel, R.M. Coates, and J. Chappell. 2005. Kinetic and molecular analysis of 5-Epiaristolochene-1,3-Dihydroxylase, a cytochrome P450 enzyme catalyzing successive hydroxylations of sesquiterpenes. Journal of Biological Chemistry 280:3686-3696.

Wu, S., M.A. Schoenbeck, B.T. Greenhagen, S. Takahashi, S. Lee, R.M. Coates, and J. Chappell. 2005. Surrogate splicing for functional analysis of sesquiterpene synthase genes. Plant Physiology 138:1322-1333.
Authors in PSS Dept.: Joseph Chappell Shuiqin Wu

Chappell, J. 2004. Valencene synthase—a biochemical magician and harbinger of transgenic aromas Trends Plant Science 9:266-269.

Okada, S., T.P. Devarenne, M. Murakami, H. Abe, and J. Chappell. 2004. Characterization of botryococcene synthase enzyme activity, a squalene synthase-like activity from the green microalga Botryococcus braunii, Race B. Archives of Biochemistry and Biophysics 422: 110-8.

O’Maille, P.E., J. Chappell, and J.P. Noel. 2004. A single-vial analytical and quantitative gas chromatography-mass spectrometry assay for terpene synthases. Analytical Biochemistry 15:210-217.

O’Maille, P.E., M.D. Tsai, B.T. Greenhagen, J. Chappell, and J.P. Noel. 2004. Gene library synthesis by structure-based combinatorial protein engineering. Methods in Enzymology 388:75-91.

Zhao, Y., D.J. Schenk, S. Takahashi, J. Chappell, and R.M. Coates. 2004. Eremophilane sesquiterpene from capsidiol. Journal Organic Chemistry 9:7428-435.

Other Research Publications

Wu, S., T. Niehaus, S. Kinison, S. Husodo, R. Williams, and J. Chappell. 2010. Engineering high value oil production. pp. 45-60. IN: B. Siminszky, ed. Tobacco. Proceedings, 64th Tobacco Science Research Conference–Tobacco Research in the Era of Biotechnology and Genomics, Hilton Head, SC, Oct. 3-6.
Authors in PSS Dept.: Joseph Chappell Thomas Niehaus Scott Kinison