Research Accomplishment Reports 2009

Genomics, Molecular Biology and Cell Biology of Sonchus yellow net virus, a Plant Rhabdovirus

M.M. Goodin
Department of Plant Pathology

 

Non-Technical Summary

This project seeks to develop vectors derived from a plant rhabdovirus, as well providing insight into the molecular events that underly the host/virus interaction. The purpose to this project is to identify host genes and proteins that are required for viral replication. A second purpose is to develop viral vectors from an engineered recombinant virus.

2009 Project Description

This project has produced eleven publications, which have significantly advanced our understanding of the cellular and molecular biology of plant-adapted rhabdoviruses. Additionally, significant resources have been generated such as novel expression vectors and transgenic plants that can be employed broadly in plant biology research. The research findings associated with this project have been disseminated via publications in high-level journals that are read by international audiences. Additionally, significant findings associated with this project have been presented at conferences and invited seminars at international, national and local research centers or universities. The resources such as plant lines and vectors have also been provided to researchers all over the world, to facilitate research in diverse areas of plant cell biology and virology. Four postdoctoral scholars, three graduate students and two undergraduate researchers received training during the course of this project.

2009 Impact

The transgenic plant lines and vectors generated during the course of this project are being used internationally in a manner that will significantly improve the quality of microscopy-based plant research. This is due to the fact that these resources permit researchers the unprecedented ability to monitor nuclear and membrane dynamics in living cells. Additionally, this our research with plant-adapted rhabdoviruses has provided insight that could lead to novel methods to develop plants that are resistant to such viruses.

2009 Publications

Senthil, G., Liu, H., Puram, V.G., Clark, A., Stromberg, A., Goodin, M.M. (2005) Specific and common changes in Nicotiana benthamiana gene expression in response to infection by enveloped viruses. J Gen Virol. 86:2615-2625.

Goodin, M., Yelton, S., Ghosh, D., Mathews, S., Lesnaw, J. (2005) Live-cell imaging of rhabdovirus-induced morphological changes in plant nuclear membranes. Mol Plant Microbe Interact. 18:703-709.

Tsai, C.W., Redinbaugh, M.G., Willie, K.J., Reed, S., Goodin, M., Hogenhout, S.A. (2005) Complete genome sequence and in planta subcellular localization of maize fine streak virus proteins. J Virol. 79:5304-5314.

Jackson, A.O., Dietzgen, R.G., Goodin, M.M., Bragg, J.N., Deng, M. (2005) Biology of Plant Rhavdoviruses. Annu Rev Phytopathol. 43:623-660.

Whitham, S.A., Yang, C., and Goodin, M.M. (2006) Global Impact: Elucidating Plant Responses to Viral Infection. Mol. Plant Plant-Mic. Interact. 19: 1207-1215.

Goodin, M.M., Chakrabarty, R., Banerjee, R., Yelton, S., DeBolt, S. (2007) Update on live-cell imaging in plant cells: New Gateways to discovery. Plant Physiology, 145:1100-1109.

Chakrabarty, C., Banerjee, R., Chung, S-M., Farman, M., Citovsky, V., Hogenhout, S.A., Tzfira, T., and Goodin, M.M. (2007) pSITE Vectors for Stable Integration or Transient Expression of Autofluorescent Protein Fusions in Plants: Probing Nicotiana benthamiana-Virus Interactions. MPMI. 20:740-750

Goodin, M.M., Chakrabarty, R., Yelton, S., Martin, K., Clark, A., Brooks, R. (2007) Membrane and protein dynamics in live plant nuclei infected with Sonchus yellow net virus, a plant-adapted rhabdovirus. J Gen Virol. 88:1810-1820.

Deng, M., Bragg, J.N., Ruzin, S., Schichnes, D., King, D., Goodin, M.M., Jackson, A.O. (2007) Role of the sonchus yellow net virus N protein in formation of nuclear viroplasms. J Virol. 81:5362-5374.