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Developing MicroRNA Vectors for Gene Suppression in Agricultural Plants
G. Tang
Department of Plant and Soil Sciences
Non-Technical Summary
Our objective is to develop high throughput tools (miRNA vectors in this project) for functional genomics of crop plants. We not only provide the optimized miRNA vectors, but also develop a workable and simplified protocol to help users to fulfill their goal to silence any gene of interest in any crop. Now the framework for this has been established.
2010 Project Description
We completed this project and conclude that the success for random RNAi in plants depends on a novel and reasonable approach for the designing of a random library. The original proposed approach must be changed for a new endeavor. We are currently working to develop a new approach to resume this project by submitting a new proposal.
2010 Impact
Our objective is to develop high throughput tools (miRNA vectors in this project) for functional genomics of crop plants. We not only provide the optimized miRNA vectors, but also develop a workable and simplified protocol to help users to fulfill their goal to silence any gene of interest in any crop. Now the framework for this has been established.
2010 Publications
Xiaoyun Jia, Wang-Xia Wang, Ligang Ren, Qi-Jun Chen, Venugopal Mendu, Benjamin Willcut, Randy Dinkins, Xiaoqing Tang, and Guiliang Tang (2010) Differential and dynamic regulation of miR398 and its targets in response to ABA and salt stress in Populus tremula and Arabidopsis thaliana. Plant Molecular Biology 71: 51-59.
Xiaoyun Jia, Ligang Ren, Qi-Jun Chen, Runzhi Li and Guiliang Tang (2010) UV-B responsive microRNAs in Populus tremula. J. of Plant Physiology 166: 2046-2057.
Ricky Lewis, Venugopal Mendu, David McNear, and Guiliang Tang (2010) Roles of microRNAs in Plant Abiotic Stress, Molecular Techniques in Crop Improvement. 2nd Edition, edited by S. Mohan Jain and D.S. Brar. Springer Netherlands, pp 357-372.
Mian Gu, Ke Xu, Aiqun Chen, Yiyong Zhu, Guiliang Tang, and Guohua Xu (2010) Expression Analysis Suggests Potential Roles of microRNAs for Phosphate and Arbuscular Mycorrhizal Signaling in Solanum lycopersicum. Physiol Plant.138(2):226-37.
Xiaoyun Jia, Venugopal Mendu, and Guiliang Tang (2010) An array platform for identification of stress-responsive miRNAs in plants, "Methods in Molecular Biology; Plant Stress Tolerance- Methods and Protocols" edited by Ramanjulu Sunkar, Oklohoma State University, Stillwater, OK, USA. HUMANA PRESS.